Renaturation of dextranase activity from culture supernatant fluids of Streptococcus sobrinus after sodium dodecylsulfate polyacrylamide gel electrophoresis

John F. Barrett, Roy Curtiss

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A rapid and reproducible method for the assay of individual fractions of the multicomponent dextranase activity of Streptococcus sobrinus after reduction/denaturation and electrophoresis in acrylamide gels is described. Multiple forms of dextranase, possible virulence factors in the formation of dental caries by oral Streptococci (S. mutans, S. sobrinus, S. sanguis, S. cricetus, and S. rattus), have been separated in sodium dodecylsulfate-polyacrylamide gels into which an indicator substrate, blue dextran, has been incorporated, and identified after renaturation to remove the reducing/denaturing agents of the Laemmli buffer system.

Original languageEnglish (US)
Pages (from-to)365-370
Number of pages6
JournalAnalytical Biochemistry
Volume158
Issue number2
DOIs
StatePublished - Nov 1 1986
Externally publishedYes

Fingerprint

Dextranase
Streptococcus sobrinus
Electrophoresis
Polyacrylamide Gel Electrophoresis
Sodium
Denaturation
Streptococcus mutans
Fluids
Acrylamide
Reducing Agents
Dental Caries
Virulence Factors
Cricetinae
Assays
Gels
Substrates
polyacrylamide gels

Keywords

  • blue dextran
  • dental caries
  • dextranase
  • gel electrophoresis
  • renaturation
  • Streptococcus sobrinus

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Renaturation of dextranase activity from culture supernatant fluids of Streptococcus sobrinus after sodium dodecylsulfate polyacrylamide gel electrophoresis. / Barrett, John F.; Curtiss, Roy.

In: Analytical Biochemistry, Vol. 158, No. 2, 01.11.1986, p. 365-370.

Research output: Contribution to journalArticle

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