Remodeling nuclear architecture allows efficient transport of herpesvirus capsids by diffusion

Jens B. Bosse, Ian B. Hogue, Marina Feric, Stephan Y. Thiberge, Beate Sodeik, Clifford P. Brangwynne, Lynn W. Enquist

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

The nuclear chromatin structure confines the movement of large macromolecular complexes to interchromatin corrals. Herpesvirus capsids of approximately 125 nm assemble in the nucleoplasm and must reach the nuclear membranes for egress. Previous studies concluded that nuclear herpesvirus capsid motility is active, directed, and based on nuclear filamentous actin, suggesting that large nuclear complexes need metabolic energy to escape nuclear entrapment. However, this hypothesis has recently been challenged. Commonly used microscopy techniques do not allow the imaging of rapid nuclear particle motility with sufficient spatiotemporal resolution. Here, we use a rotating, oblique light sheet, which we dubbed a ring-sheet, to image and track viral capsids with high temporal and spatial resolution. We do not find any evidence for directed transport. Instead, infection with different herpesviruses induced an enlargement of interchromatin domains and allowed particles to diffuse unrestricted over longer distances, thereby facilitating nuclear egress for a larger fraction of capsids.

Original languageEnglish (US)
Pages (from-to)E5752-E5733
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number42
DOIs
StatePublished - Oct 20 2015
Externally publishedYes

Keywords

  • Capsid
  • Herpes
  • Light sheet
  • Nucleus
  • Ring sheet

ASJC Scopus subject areas

  • General

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