TY - JOUR
T1 - Remediation of petroleum hydrocarbon-contaminated sites by DNA diagnosis-based bioslurping technology
AU - Kim, Seungjin
AU - Krajmalnik-Brown, Rosa
AU - Kim, Jong Oh
AU - Chung, Jinwook
N1 - Funding Information:
This study was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean Government ( NRF-2013R1A2A1A09007252 ).
Publisher Copyright:
© 2014 Elsevier B.V.
Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 2014
Y1 - 2014
N2 - The application of effective remediation technologies can benefit from adequate preliminary testing, such as in lab-scale and Pilot-scale systems. Bioremediation technologies have demonstrated tremendous potential with regards to cost, but they cannot be used for all contaminated sites due to limitations in biological activity. The purpose of this study was to develop a DNA diagnostic method that reduces the time to select contaminated sites that are good candidates for bioremediation. We applied an oligonucleotide microarray method to detect and monitor genes that lead to aliphatic and aromatic degradation. Further, the bioremediation of a contaminated site, selected based on the results of the genetic diagnostic method, was achieved successfully by applying bioslurping in field tests. This gene-based diagnostic technique is a powerful tool to evaluate the potential for bioremediation in petroleum hydrocarbon contaminated soil.
AB - The application of effective remediation technologies can benefit from adequate preliminary testing, such as in lab-scale and Pilot-scale systems. Bioremediation technologies have demonstrated tremendous potential with regards to cost, but they cannot be used for all contaminated sites due to limitations in biological activity. The purpose of this study was to develop a DNA diagnostic method that reduces the time to select contaminated sites that are good candidates for bioremediation. We applied an oligonucleotide microarray method to detect and monitor genes that lead to aliphatic and aromatic degradation. Further, the bioremediation of a contaminated site, selected based on the results of the genetic diagnostic method, was achieved successfully by applying bioslurping in field tests. This gene-based diagnostic technique is a powerful tool to evaluate the potential for bioremediation in petroleum hydrocarbon contaminated soil.
KW - BTEX
KW - Bioslurping
KW - Diagnosis technology
KW - Oligonucleotide microarray
KW - TPH
UR - http://www.scopus.com/inward/record.url?scp=84906667978&partnerID=8YFLogxK
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U2 - 10.1016/j.scitotenv.2014.08.002
DO - 10.1016/j.scitotenv.2014.08.002
M3 - Article
C2 - 25129160
AN - SCOPUS:84906667978
SN - 0048-9697
VL - 497-498
SP - 250
EP - 259
JO - Science of the Total Environment
JF - Science of the Total Environment
ER -