Remediation of petroleum hydrocarbon-contaminated sites by DNA diagnosis-based bioslurping technology

Seungjin Kim, Rosa Krajmalnik-Brown, Jong Oh Kim, Jinwook Chung

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The application of effective remediation technologies can benefit from adequate preliminary testing, such as in lab-scale and Pilot-scale systems. Bioremediation technologies have demonstrated tremendous potential with regards to cost, but they cannot be used for all contaminated sites due to limitations in biological activity. The purpose of this study was to develop a DNA diagnostic method that reduces the time to select contaminated sites that are good candidates for bioremediation. We applied an oligonucleotide microarray method to detect and monitor genes that lead to aliphatic and aromatic degradation. Further, the bioremediation of a contaminated site, selected based on the results of the genetic diagnostic method, was achieved successfully by applying bioslurping in field tests. This gene-based diagnostic technique is a powerful tool to evaluate the potential for bioremediation in petroleum hydrocarbon contaminated soil.

Original languageEnglish (US)
Pages (from-to)250-259
Number of pages10
JournalScience of the Total Environment
Volume497
DOIs
StatePublished - 2014

Fingerprint

Bioremediation
Petroleum
petroleum hydrocarbon
Hydrocarbons
Remediation
bioremediation
remediation
DNA
Crude oil
Genes
gene
Oligonucleotides
Microarrays
Bioactivity
Soils
Degradation
degradation
Testing
cost
method

Keywords

  • Bioslurping
  • BTEX
  • Diagnosis technology
  • Oligonucleotide microarray
  • TPH

ASJC Scopus subject areas

  • Environmental Chemistry
  • Pollution
  • Waste Management and Disposal
  • Environmental Engineering

Cite this

Remediation of petroleum hydrocarbon-contaminated sites by DNA diagnosis-based bioslurping technology. / Kim, Seungjin; Krajmalnik-Brown, Rosa; Kim, Jong Oh; Chung, Jinwook.

In: Science of the Total Environment, Vol. 497, 2014, p. 250-259.

Research output: Contribution to journalArticle

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