TY - JOUR
T1 - Reliability of 3 urine specific gravity meters for measuring Brix and urine solutions at different temperatures
AU - Wardenaar, Floris
AU - Ortega-Santos, Carmen P.
AU - Vento, Kaila
AU - Olzinski, Stephanie
AU - Olig, Jason
AU - Kavouras, Stavros
AU - Johnston, Carol
N1 - Publisher Copyright:
© by the National Athletic Trainers’ Association, Inc
PY - 2021/4
Y1 - 2021/4
N2 - Context: Urine specific gravity (USG) should be measured at room temperature (20°C), but the temperature of the sample is not always considered. Objective: To evaluate the effect of sample temperature on the measurement accuracy of a digital refractometer (DIG), manual optical refractometer (MAN), and hydrometer (HYD). Design: Descriptive laboratory study. Main Outcome Measure(s): Urine specific gravity. Results: Experiment 1 (24 Brix (°Bx) samples) showed that measurements via the DIG and MAN did not differ from the reference, but HYD provided lower or inconsistent values compared with °Bx and was highly correlated with 8Bx solutions (r, > = 0.89). The overall diagnostic ability of elevated USG cutoff values (≥1.020, ≥1.025, ≥1.030) was high for all tools (area under the curve .0.92). Misclassification of samples increased from 0 to 2 at 1.020 to 1 to 3 samples at cutoffs of 1.025 and 1.030 USG. Bland-Altman analysis showed that the DIG 5°C underreported slightly without reporting bias (r = -0.344, P = .13); all other plots for the DIG, MAN, and HYD showed considerably larger underreporting at higher concentrations (r = -0.21 to -0.97 with P >.02) at all temperatures. The outcomes of experiment 2 (33 fresh urine samples) using DIG 20°C as the standard demonstrated only negligible differences between the DIG and MAN at all temperatures but larger differences using the HYD. Conclusions: All tools showed reporting bias compared with the °Bx solutions, which can affect the classification of low and high urine concentration at higher USG cutoff values, especially with a sample temperature of 37°C.
AB - Context: Urine specific gravity (USG) should be measured at room temperature (20°C), but the temperature of the sample is not always considered. Objective: To evaluate the effect of sample temperature on the measurement accuracy of a digital refractometer (DIG), manual optical refractometer (MAN), and hydrometer (HYD). Design: Descriptive laboratory study. Main Outcome Measure(s): Urine specific gravity. Results: Experiment 1 (24 Brix (°Bx) samples) showed that measurements via the DIG and MAN did not differ from the reference, but HYD provided lower or inconsistent values compared with °Bx and was highly correlated with 8Bx solutions (r, > = 0.89). The overall diagnostic ability of elevated USG cutoff values (≥1.020, ≥1.025, ≥1.030) was high for all tools (area under the curve .0.92). Misclassification of samples increased from 0 to 2 at 1.020 to 1 to 3 samples at cutoffs of 1.025 and 1.030 USG. Bland-Altman analysis showed that the DIG 5°C underreported slightly without reporting bias (r = -0.344, P = .13); all other plots for the DIG, MAN, and HYD showed considerably larger underreporting at higher concentrations (r = -0.21 to -0.97 with P >.02) at all temperatures. The outcomes of experiment 2 (33 fresh urine samples) using DIG 20°C as the standard demonstrated only negligible differences between the DIG and MAN at all temperatures but larger differences using the HYD. Conclusions: All tools showed reporting bias compared with the °Bx solutions, which can affect the classification of low and high urine concentration at higher USG cutoff values, especially with a sample temperature of 37°C.
KW - Accuracy
KW - Hydration status
KW - Validation
UR - http://www.scopus.com/inward/record.url?scp=85105051527&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85105051527&partnerID=8YFLogxK
U2 - 10.4085/1062-6050-0142.20
DO - 10.4085/1062-6050-0142.20
M3 - Article
C2 - 33788928
AN - SCOPUS:85105051527
SN - 1062-6050
VL - 56
SP - 389
EP - 395
JO - Journal of Athletic Training
JF - Journal of Athletic Training
IS - 4
ER -