Regulation of hexokinase II activity and expression in human muscle by moderate exercise

Janice A. Koval, Ralph A. DeFronzo, Robert M. O'Doherty, Richard Printz, Hossein Ardehali, Daryl K. Granner, Lawrence J. Mandarino

Research output: Contribution to journalArticle

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Abstract

A single bout of exercise increases the rate of insulin-stimulated glucose uptake and metabolism in skeletal muscle. Exercise also increases insulin-stimulated glucose 6-phosphate in skeletal muscle, suggesting that exercise increases hexokinase activity. Within 3 h, exercise increases hexokinase II (HK II) mRNA and activity in skeletal muscle from rats. It is not known, however, if a single bout of moderate-intensity exercise increases HK II expression in humans. The present study was undertaken to answer this question. Six subjects had percutaneous biopsies of the vastus lateralis muscle before and 3 h after a single 3-h session of moderate-intensity aerobic (60% of maximal oxygen consumption) exercise. Glycogen synthase, HK I, and HK II activities as well as HK I and HK II mRNA content were determined from the muscle biopsy specimens. The fractional velocity of glycogen synthase was increased by 446 ± 84% after exercise (P < 0.005). Hexokinase II activity in the soluble fraction of the homogenates increased from 1.2 ± 0.4 to 4.5 ± 1.6 pmol · min-1 · μg-1 (P < 0.05) but was unchanged in the particulate fraction (4.3 ± 1.3 vs. 5.3 ± 1.5). HK I activity in neither the soluble nor particulate fraction changed after exercise. Relative to a 28S rRNA control signal, HK II mRNA increased from 0.091 ± 0.02 to 0.195 ± 0.037 (P < 0.05), whereas HK I mRNA was unchanged (0.414 ± 0.061 vs. 0.498 ± 0.134, P < 0.20). The increase in HK II activity after moderate exercise in healthy subjects could be one factor responsible for the enhanced rate of insulin-stimulated glucose uptake seen after exercise.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume274
Issue number2 37-2
StatePublished - Feb 1998
Externally publishedYes

Fingerprint

Hexokinase
Muscle
Exercise
Muscles
Glycogen Synthase
Messenger RNA
Biopsy
Skeletal Muscle
Insulin
Glucose
Glucose-6-Phosphate
Quadriceps Muscle
Metabolism
Rats
Oxygen Consumption
Healthy Volunteers
Oxygen

Keywords

  • Exercise
  • Glucose uptake
  • Hexokinase
  • Skeletal muscle

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry
  • Physiology (medical)

Cite this

Koval, J. A., DeFronzo, R. A., O'Doherty, R. M., Printz, R., Ardehali, H., Granner, D. K., & Mandarino, L. J. (1998). Regulation of hexokinase II activity and expression in human muscle by moderate exercise. American Journal of Physiology - Endocrinology and Metabolism, 274(2 37-2).

Regulation of hexokinase II activity and expression in human muscle by moderate exercise. / Koval, Janice A.; DeFronzo, Ralph A.; O'Doherty, Robert M.; Printz, Richard; Ardehali, Hossein; Granner, Daryl K.; Mandarino, Lawrence J.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 274, No. 2 37-2, 02.1998.

Research output: Contribution to journalArticle

Koval, JA, DeFronzo, RA, O'Doherty, RM, Printz, R, Ardehali, H, Granner, DK & Mandarino, LJ 1998, 'Regulation of hexokinase II activity and expression in human muscle by moderate exercise', American Journal of Physiology - Endocrinology and Metabolism, vol. 274, no. 2 37-2.
Koval JA, DeFronzo RA, O'Doherty RM, Printz R, Ardehali H, Granner DK et al. Regulation of hexokinase II activity and expression in human muscle by moderate exercise. American Journal of Physiology - Endocrinology and Metabolism. 1998 Feb;274(2 37-2).
Koval, Janice A. ; DeFronzo, Ralph A. ; O'Doherty, Robert M. ; Printz, Richard ; Ardehali, Hossein ; Granner, Daryl K. ; Mandarino, Lawrence J. / Regulation of hexokinase II activity and expression in human muscle by moderate exercise. In: American Journal of Physiology - Endocrinology and Metabolism. 1998 ; Vol. 274, No. 2 37-2.
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