Regulation of capsular polysaccharide synthesis in Escherichia coli K12

S. Gottesman, V. Stout

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

Synthesis of the capsular polysaccharide colanic acid in Escherichia coli K12 is regulated by a complex network of regulatory proteins. This regulation is expressed at the level of transcription of the cps (capsular polysaccharide synthesis) genes. Two positive regulators, RcsA and RcsB, are necessary for maximal capsule expression. The availability of RcsA is normally limited because the RcsA protein is rapidly degraded by the Lon ATP-dependent protease. There-fore Lon acts, indirectly, as a negative regulator of capsule synthesis. The sequence predicted for RcsB suggests that it is the effector component of a two-component system; a protein with homology to sensors, RcsC, also plays a role in capsule regulation. We propose a model for capsule synthesis in which RcsA interacts with RcsB to stimulate transcription of the cps genes. The mechanism of regulation of colanic acid synthesis in E. coli may apply to other capsules in a variety of Gram-negative bacteria.

Original languageEnglish (US)
Pages (from-to)1599-1606
Number of pages8
JournalMolecular Microbiology
Volume5
Issue number7
StatePublished - Jul 1991
Externally publishedYes

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Escherichia coli K12
Capsules
Polysaccharides
Protease La
ATP-Dependent Proteases
Proteins
Gram-Negative Bacteria
Genes
Escherichia coli

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Regulation of capsular polysaccharide synthesis in Escherichia coli K12. / Gottesman, S.; Stout, V.

In: Molecular Microbiology, Vol. 5, No. 7, 07.1991, p. 1599-1606.

Research output: Contribution to journalArticle

Gottesman, S & Stout, V 1991, 'Regulation of capsular polysaccharide synthesis in Escherichia coli K12', Molecular Microbiology, vol. 5, no. 7, pp. 1599-1606.
Gottesman, S. ; Stout, V. / Regulation of capsular polysaccharide synthesis in Escherichia coli K12. In: Molecular Microbiology. 1991 ; Vol. 5, No. 7. pp. 1599-1606.
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