Regulated expression of functional external guide sequences in mammalian cells using a U6 RNA polymerase III promoter

Elizaveta Kovrigina, Li Yang, Emmanuel Pfund, Sidney Altman

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

A regulatable promoter has been stably integrated into a human embryonic kidney cell line. The promoter is a pol III mouse promoter and is under the control of ponasterone A, an ecdysone inducer. The promoter controls transcription of an external guide sequence (EGS) targeted against Rpp38, a protein subunit of human RNase P, or of lamin A/C, a gene product located in the nucleus. The amounts of protein of both gene products are severely reduced when the EGSs are made. Several other, but not all, of the protein subunits of RNase P are also inhibited in both mRNA and protein levels when Rpp38 mRNA is targeted. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish (US)
Pages (from-to)1588-1595
Number of pages8
JournalRNA
Volume11
Issue number10
DOIs
StatePublished - Oct 2005

Keywords

  • Inhibition of gene expression
  • Lamin A/C
  • RNase P
  • Rpp38

ASJC Scopus subject areas

  • Molecular Biology

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