Regional uptake of meal fatty acids in humans

Michael D. Jensen, Michael G. Sarr, Daniel A. Dumesic, Peter A. Southorn, James A. Levine

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Two protocols were performed to study meal fatty acid metabolism. In protocol 1, 14 patients scheduled for elective intra-abdominal surgery (11 undergoing bariatric surgery for severe obesity) consumed a meal containing [3H]triolein in the evening before surgery. This allowed us to measure adipose tissue lipid specific activity (SA) in mesenteric and omental, deep and superficial abdominal subcutaneous adipose tissue. Intra-abdominal adipose tissue lipid SA was greater than subcutaneous lipid SA. There were no significant differences between mesenteric and omental or between deep and superficial abdominal subcutaneous adipose tissue. In protocol 2, meal fatty acid oxidation and uptake into subcutaneous and omental adipose tissue ([ 3H]triolein) were measured in six normal, healthy volunteers. Meal fatty acid oxidation (3H2O generation) plus that remaining in plasma (∼1%) plus uptake into upper body subcutaneous, lower body subcutaneous, and visceral fat allowed us to account for 98 ± 6% of meal fatty acids 24 h after meal ingestion. We conclude that omental fat is a good surrogate for visceral fat and that abdominal subcutaneous fat depots are comparable with regard to meal fatty acid metabolic studies. Using [ 3H]triolein, we were able to account for virtually 100% of meal fatty acids 24 h after meal ingestion. These results support the meal fatty acid tracer model as a way to study the metabolic fate of dietary fat.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume285
Issue number6 48-6
StatePublished - Dec 2003
Externally publishedYes

Fingerprint

Meals
Fatty Acids
Triolein
Tissue
Fats
Abdominal Subcutaneous Fat
Surgery
Intra-Abdominal Fat
Lipids
Subcutaneous Fat
Oxidation
Healthy Volunteers
Dietary Fats
Eating
Metabolism
Bariatric Surgery
Morbid Obesity
Plasmas
Adipose Tissue

Keywords

  • [H]triolein
  • Adipose tissue
  • Body composition
  • Obesity

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry

Cite this

Jensen, M. D., Sarr, M. G., Dumesic, D. A., Southorn, P. A., & Levine, J. A. (2003). Regional uptake of meal fatty acids in humans. American Journal of Physiology - Endocrinology and Metabolism, 285(6 48-6).

Regional uptake of meal fatty acids in humans. / Jensen, Michael D.; Sarr, Michael G.; Dumesic, Daniel A.; Southorn, Peter A.; Levine, James A.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 285, No. 6 48-6, 12.2003.

Research output: Contribution to journalArticle

Jensen, MD, Sarr, MG, Dumesic, DA, Southorn, PA & Levine, JA 2003, 'Regional uptake of meal fatty acids in humans', American Journal of Physiology - Endocrinology and Metabolism, vol. 285, no. 6 48-6.
Jensen MD, Sarr MG, Dumesic DA, Southorn PA, Levine JA. Regional uptake of meal fatty acids in humans. American Journal of Physiology - Endocrinology and Metabolism. 2003 Dec;285(6 48-6).
Jensen, Michael D. ; Sarr, Michael G. ; Dumesic, Daniel A. ; Southorn, Peter A. ; Levine, James A. / Regional uptake of meal fatty acids in humans. In: American Journal of Physiology - Endocrinology and Metabolism. 2003 ; Vol. 285, No. 6 48-6.
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