Regional assignment of the gene for human liver/bone/kidney alkaline phosphatase to chromosome 1p36.1-p34

Moyra Smith, Mitchell J. Weiss, Constance A. Griffin, Jeffrey C. Murray, Kenneth H. Buetow, Beverly S. Emanuel, Paula S. Henthorn, Harry Harris

Research output: Contribution to journalArticlepeer-review

93 Scopus citations

Abstract

We have used three different methods to map the human liver/bone/kidney alkaline phosphatase (ALPL) locus: (1) Southern blot analysis of DNA derived from a panel of human-rodent somatic cell hybrids; (2) in situ hybridization to human chromosomes; and (3) genetic linkage analysis. Our results indicate that the ALPL locus maps to human chromosome bands 1p36.1-p34 and is genetically linked to the Rh (maximum lod score of 15.66 at a recombination value of 0.10) and fucosidase A (maximum lod score of 8.24 at a recombination value of 0.02) loci. These results, combined with restriction fragment length polymorphisms identified by ALPL DNA probes, provide a useful marker for gene mapping studies involving the short arm of chromosome 1. In addition, our results help to elucidate further the structure and evolution of the human alkaline phosphatase multigene enzyme family.

Original languageEnglish (US)
Pages (from-to)139-143
Number of pages5
JournalGenomics
Volume2
Issue number2
DOIs
StatePublished - Feb 1988

ASJC Scopus subject areas

  • Genetics

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