Regional assignment of the gene for human liver/bone/kidney alkaline phosphatase to chromosome 1p36.1-p34

Moyra Smith; Mitchell J. Weiss; Constance A. Griffin; Jeffrey C. Murray; Kenneth H. Buetow; Beverly S. Emanuel; Paula S. Henthorn; Harry Harris

doi:10.1016/0888-7543(88)90095-X

Regional assignment of the gene for human liver/bone/kidney alkaline phosphatase to chromosome 1p36.1-p34

Moyra Smith, Mitchell J. Weiss, Constance A. Griffin, Jeffrey C. Murray, Kenneth H. Buetow, Beverly S. Emanuel, Paula S. Henthorn, Harry Harris

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103 Scopus citations

Abstract

We have used three different methods to map the human liver/bone/kidney alkaline phosphatase (ALPL) locus: (1) Southern blot analysis of DNA derived from a panel of human-rodent somatic cell hybrids; (2) in situ hybridization to human chromosomes; and (3) genetic linkage analysis. Our results indicate that the ALPL locus maps to human chromosome bands 1p36.1-p34 and is genetically linked to the Rh (maximum lod score of 15.66 at a recombination value of 0.10) and fucosidase A (maximum lod score of 8.24 at a recombination value of 0.02) loci. These results, combined with restriction fragment length polymorphisms identified by ALPL DNA probes, provide a useful marker for gene mapping studies involving the short arm of chromosome 1. In addition, our results help to elucidate further the structure and evolution of the human alkaline phosphatase multigene enzyme family.

Original language	English (US)
Pages (from-to)	139-143
Number of pages	5
Journal	Genomics
Volume	2
Issue number	2
DOIs	https://doi.org/10.1016/0888-7543(88)90095-X
State	Published - Feb 1988
Externally published	Yes

ASJC Scopus subject areas

Genetics

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@article{75e05b1fb2a54ce4a08ecd863ef7e29a,

title = "Regional assignment of the gene for human liver/bone/kidney alkaline phosphatase to chromosome 1p36.1-p34",

abstract = "We have used three different methods to map the human liver/bone/kidney alkaline phosphatase (ALPL) locus: (1) Southern blot analysis of DNA derived from a panel of human-rodent somatic cell hybrids; (2) in situ hybridization to human chromosomes; and (3) genetic linkage analysis. Our results indicate that the ALPL locus maps to human chromosome bands 1p36.1-p34 and is genetically linked to the Rh (maximum lod score of 15.66 at a recombination value of 0.10) and fucosidase A (maximum lod score of 8.24 at a recombination value of 0.02) loci. These results, combined with restriction fragment length polymorphisms identified by ALPL DNA probes, provide a useful marker for gene mapping studies involving the short arm of chromosome 1. In addition, our results help to elucidate further the structure and evolution of the human alkaline phosphatase multigene enzyme family.",

author = "Moyra Smith and Weiss, {Mitchell J.} and Griffin, {Constance A.} and Murray, {Jeffrey C.} and Buetow, {Kenneth H.} and Emanuel, {Beverly S.} and Henthorn, {Paula S.} and Harry Harris",

note = "Funding Information: We thank Robert Nussbaum for the gift of genomic DNA from somatic cell hybrids MR 8-l and MR 8-2, Anita Hawkins and Catherine DeHaven for technical assistance, and Max Miinke for critical reading of this manuscript. This work was supported by Grants NIH GM 27018 and March of Dimes 858 (to H.H.), NIH Medical Scientist Training Program T32-GM07170 (for M.W.), NIH Training Grant CA09485-01 (for C.G.), NIH GM 32592 (to B.E.), NIH HD20998 (for J.M.), and the NIAAA Intramural Research Program (for M.S.).",

year = "1988",

month = feb,

doi = "10.1016/0888-7543(88)90095-X",

language = "English (US)",

volume = "2",

pages = "139--143",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press Inc.",

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TY - JOUR

T1 - Regional assignment of the gene for human liver/bone/kidney alkaline phosphatase to chromosome 1p36.1-p34

AU - Smith, Moyra

AU - Weiss, Mitchell J.

AU - Griffin, Constance A.

AU - Murray, Jeffrey C.

AU - Buetow, Kenneth H.

AU - Emanuel, Beverly S.

AU - Henthorn, Paula S.

AU - Harris, Harry

N1 - Funding Information: We thank Robert Nussbaum for the gift of genomic DNA from somatic cell hybrids MR 8-l and MR 8-2, Anita Hawkins and Catherine DeHaven for technical assistance, and Max Miinke for critical reading of this manuscript. This work was supported by Grants NIH GM 27018 and March of Dimes 858 (to H.H.), NIH Medical Scientist Training Program T32-GM07170 (for M.W.), NIH Training Grant CA09485-01 (for C.G.), NIH GM 32592 (to B.E.), NIH HD20998 (for J.M.), and the NIAAA Intramural Research Program (for M.S.).

PY - 1988/2

Y1 - 1988/2

N2 - We have used three different methods to map the human liver/bone/kidney alkaline phosphatase (ALPL) locus: (1) Southern blot analysis of DNA derived from a panel of human-rodent somatic cell hybrids; (2) in situ hybridization to human chromosomes; and (3) genetic linkage analysis. Our results indicate that the ALPL locus maps to human chromosome bands 1p36.1-p34 and is genetically linked to the Rh (maximum lod score of 15.66 at a recombination value of 0.10) and fucosidase A (maximum lod score of 8.24 at a recombination value of 0.02) loci. These results, combined with restriction fragment length polymorphisms identified by ALPL DNA probes, provide a useful marker for gene mapping studies involving the short arm of chromosome 1. In addition, our results help to elucidate further the structure and evolution of the human alkaline phosphatase multigene enzyme family.

AB - We have used three different methods to map the human liver/bone/kidney alkaline phosphatase (ALPL) locus: (1) Southern blot analysis of DNA derived from a panel of human-rodent somatic cell hybrids; (2) in situ hybridization to human chromosomes; and (3) genetic linkage analysis. Our results indicate that the ALPL locus maps to human chromosome bands 1p36.1-p34 and is genetically linked to the Rh (maximum lod score of 15.66 at a recombination value of 0.10) and fucosidase A (maximum lod score of 8.24 at a recombination value of 0.02) loci. These results, combined with restriction fragment length polymorphisms identified by ALPL DNA probes, provide a useful marker for gene mapping studies involving the short arm of chromosome 1. In addition, our results help to elucidate further the structure and evolution of the human alkaline phosphatase multigene enzyme family.

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Regional assignment of the gene for human liver/bone/kidney alkaline phosphatase to chromosome 1p36.1-p34

Abstract

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