Regional assignment of the gene for human liver/bone/kidney alkaline phosphatase to chromosome 1p36.1-p34

Moyra Smith, Mitchell J. Weiss, Constance A. Griffin, Jeffrey C. Murray, Kenneth Buetow, Beverly S. Emanuel, Paula S. Henthorn, Harry Harris

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

We have used three different methods to map the human liver/bone/kidney alkaline phosphatase (ALPL) locus: (1) Southern blot analysis of DNA derived from a panel of human-rodent somatic cell hybrids; (2) in situ hybridization to human chromosomes; and (3) genetic linkage analysis. Our results indicate that the ALPL locus maps to human chromosome bands 1p36.1-p34 and is genetically linked to the Rh (maximum lod score of 15.66 at a recombination value of 0.10) and fucosidase A (maximum lod score of 8.24 at a recombination value of 0.02) loci. These results, combined with restriction fragment length polymorphisms identified by ALPL DNA probes, provide a useful marker for gene mapping studies involving the short arm of chromosome 1. In addition, our results help to elucidate further the structure and evolution of the human alkaline phosphatase multigene enzyme family.

Original languageEnglish (US)
Pages (from-to)139-143
Number of pages5
JournalGenomics
Volume2
Issue number2
DOIs
StatePublished - 1988
Externally publishedYes

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Lod Score
Human Chromosomes
Genetic Recombination
Chromosomes
alpha-L-Fucosidase
Genetic Linkage
Hybrid Cells
Chromosome Mapping
Chromosomes, Human, Pair 1
DNA Probes
Multigene Family
Southern Blotting
Restriction Fragment Length Polymorphisms
Genes
In Situ Hybridization
Alkaline Phosphatase
Rodentia
DNA
Enzymes
human ALPL protein

ASJC Scopus subject areas

  • Genetics

Cite this

Smith, M., Weiss, M. J., Griffin, C. A., Murray, J. C., Buetow, K., Emanuel, B. S., ... Harris, H. (1988). Regional assignment of the gene for human liver/bone/kidney alkaline phosphatase to chromosome 1p36.1-p34. Genomics, 2(2), 139-143. https://doi.org/10.1016/0888-7543(88)90095-X

Regional assignment of the gene for human liver/bone/kidney alkaline phosphatase to chromosome 1p36.1-p34. / Smith, Moyra; Weiss, Mitchell J.; Griffin, Constance A.; Murray, Jeffrey C.; Buetow, Kenneth; Emanuel, Beverly S.; Henthorn, Paula S.; Harris, Harry.

In: Genomics, Vol. 2, No. 2, 1988, p. 139-143.

Research output: Contribution to journalArticle

Smith, M, Weiss, MJ, Griffin, CA, Murray, JC, Buetow, K, Emanuel, BS, Henthorn, PS & Harris, H 1988, 'Regional assignment of the gene for human liver/bone/kidney alkaline phosphatase to chromosome 1p36.1-p34', Genomics, vol. 2, no. 2, pp. 139-143. https://doi.org/10.1016/0888-7543(88)90095-X
Smith, Moyra ; Weiss, Mitchell J. ; Griffin, Constance A. ; Murray, Jeffrey C. ; Buetow, Kenneth ; Emanuel, Beverly S. ; Henthorn, Paula S. ; Harris, Harry. / Regional assignment of the gene for human liver/bone/kidney alkaline phosphatase to chromosome 1p36.1-p34. In: Genomics. 1988 ; Vol. 2, No. 2. pp. 139-143.
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