Abstract

Fully understanding the metabolism of SRB provides fundamental guidelines for allowing the microorganisms to provide more beneficial services in water treatment and resource recovery. The electron-transfer pathway of sulfate respiration by Desulfovibrio vulgaris is well studied, but still partly unresolved. Here we provide deeper insight by comprehensively monitoring metabolite changes during D. vulgaris metabolism with two electron donors, lactate and pyruvate, in presence or absence of citrate-chelated soluble FeIII as an additional competing electron acceptor. H2 was produced from lactate oxidation to pyruvate, but pyruvate oxidation produced mostly formate. Accumulation of lactate-originated H2 during lag phases inhibited pyruvate transformation to acetate. Sulfate reduction was initiated by lactate-originated H2, but MQ-mediated e flow initiated sulfate reduction without delay when pyruvate was the donor. When H2-induced electron flow gave priority to FeIII reduction over sulfate reduction, the long lag phase before sulfate reduction shortened the time for iron-sulfide crystallite growth and led to smaller mackinawite (Fe1+xS) nanocrystallites. Synthesizing all the results, we propose that electron flow from lactate or pyruvate towards SO4 2− reduction to H2S are through at least three routes that are regulated by the e donor (lactate or pyruvate) and the presence or absence of another e acceptor (FeIII here). These routes are not competing, but complementary: e.g., H2 or formate production and oxidation were necessary for sulfite and disulfide/trisulfide reduction to sulfide. Our study suggests that the e donor provides a practical tool to regulate and optimize SRB-predominant bioremediation systems.

Original languageEnglish (US)
Pages (from-to)91-101
Number of pages11
JournalWater Research
Volume119
DOIs
StatePublished - Aug 1 2017

Fingerprint

crystallization
Crystallization
sulfate
electron
Electrons
Metabolism
oxidation
Oxidation
metabolism
mackinawite
Nanocrystallites
Bioremediation
iron sulfide
sulfite
Metabolites
Sulfates
Water resources
Water treatment
bioremediation
Microorganisms

Keywords

  • Crystallization
  • D. vulgaris
  • Iron-sulfide
  • SO reduction
  • Soluble Fe reduction

ASJC Scopus subject areas

  • Ecological Modeling
  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution

Cite this

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title = "Reductive precipitation of sulfate and soluble Fe(III) by Desulfovibrio vulgaris: Electron donor regulates intracellular electron flow and nano-FeS crystallization",
abstract = "Fully understanding the metabolism of SRB provides fundamental guidelines for allowing the microorganisms to provide more beneficial services in water treatment and resource recovery. The electron-transfer pathway of sulfate respiration by Desulfovibrio vulgaris is well studied, but still partly unresolved. Here we provide deeper insight by comprehensively monitoring metabolite changes during D. vulgaris metabolism with two electron donors, lactate and pyruvate, in presence or absence of citrate-chelated soluble FeIII as an additional competing electron acceptor. H2 was produced from lactate oxidation to pyruvate, but pyruvate oxidation produced mostly formate. Accumulation of lactate-originated H2 during lag phases inhibited pyruvate transformation to acetate. Sulfate reduction was initiated by lactate-originated H2, but MQ-mediated e− flow initiated sulfate reduction without delay when pyruvate was the donor. When H2-induced electron flow gave priority to FeIII reduction over sulfate reduction, the long lag phase before sulfate reduction shortened the time for iron-sulfide crystallite growth and led to smaller mackinawite (Fe1+xS) nanocrystallites. Synthesizing all the results, we propose that electron flow from lactate or pyruvate towards SO4 2− reduction to H2S are through at least three routes that are regulated by the e− donor (lactate or pyruvate) and the presence or absence of another e− acceptor (FeIII here). These routes are not competing, but complementary: e.g., H2 or formate production and oxidation were necessary for sulfite and disulfide/trisulfide reduction to sulfide. Our study suggests that the e− donor provides a practical tool to regulate and optimize SRB-predominant bioremediation systems.",
keywords = "Crystallization, D. vulgaris, Iron-sulfide, SO reduction, Soluble Fe reduction",
author = "Chen Zhou and Yun Zhou and Bruce Rittmann",
year = "2017",
month = "8",
day = "1",
doi = "10.1016/j.watres.2017.04.044",
language = "English (US)",
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pages = "91--101",
journal = "Water Research",
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TY - JOUR

T1 - Reductive precipitation of sulfate and soluble Fe(III) by Desulfovibrio vulgaris

T2 - Electron donor regulates intracellular electron flow and nano-FeS crystallization

AU - Zhou, Chen

AU - Zhou, Yun

AU - Rittmann, Bruce

PY - 2017/8/1

Y1 - 2017/8/1

N2 - Fully understanding the metabolism of SRB provides fundamental guidelines for allowing the microorganisms to provide more beneficial services in water treatment and resource recovery. The electron-transfer pathway of sulfate respiration by Desulfovibrio vulgaris is well studied, but still partly unresolved. Here we provide deeper insight by comprehensively monitoring metabolite changes during D. vulgaris metabolism with two electron donors, lactate and pyruvate, in presence or absence of citrate-chelated soluble FeIII as an additional competing electron acceptor. H2 was produced from lactate oxidation to pyruvate, but pyruvate oxidation produced mostly formate. Accumulation of lactate-originated H2 during lag phases inhibited pyruvate transformation to acetate. Sulfate reduction was initiated by lactate-originated H2, but MQ-mediated e− flow initiated sulfate reduction without delay when pyruvate was the donor. When H2-induced electron flow gave priority to FeIII reduction over sulfate reduction, the long lag phase before sulfate reduction shortened the time for iron-sulfide crystallite growth and led to smaller mackinawite (Fe1+xS) nanocrystallites. Synthesizing all the results, we propose that electron flow from lactate or pyruvate towards SO4 2− reduction to H2S are through at least three routes that are regulated by the e− donor (lactate or pyruvate) and the presence or absence of another e− acceptor (FeIII here). These routes are not competing, but complementary: e.g., H2 or formate production and oxidation were necessary for sulfite and disulfide/trisulfide reduction to sulfide. Our study suggests that the e− donor provides a practical tool to regulate and optimize SRB-predominant bioremediation systems.

AB - Fully understanding the metabolism of SRB provides fundamental guidelines for allowing the microorganisms to provide more beneficial services in water treatment and resource recovery. The electron-transfer pathway of sulfate respiration by Desulfovibrio vulgaris is well studied, but still partly unresolved. Here we provide deeper insight by comprehensively monitoring metabolite changes during D. vulgaris metabolism with two electron donors, lactate and pyruvate, in presence or absence of citrate-chelated soluble FeIII as an additional competing electron acceptor. H2 was produced from lactate oxidation to pyruvate, but pyruvate oxidation produced mostly formate. Accumulation of lactate-originated H2 during lag phases inhibited pyruvate transformation to acetate. Sulfate reduction was initiated by lactate-originated H2, but MQ-mediated e− flow initiated sulfate reduction without delay when pyruvate was the donor. When H2-induced electron flow gave priority to FeIII reduction over sulfate reduction, the long lag phase before sulfate reduction shortened the time for iron-sulfide crystallite growth and led to smaller mackinawite (Fe1+xS) nanocrystallites. Synthesizing all the results, we propose that electron flow from lactate or pyruvate towards SO4 2− reduction to H2S are through at least three routes that are regulated by the e− donor (lactate or pyruvate) and the presence or absence of another e− acceptor (FeIII here). These routes are not competing, but complementary: e.g., H2 or formate production and oxidation were necessary for sulfite and disulfide/trisulfide reduction to sulfide. Our study suggests that the e− donor provides a practical tool to regulate and optimize SRB-predominant bioremediation systems.

KW - Crystallization

KW - D. vulgaris

KW - Iron-sulfide

KW - SO reduction

KW - Soluble Fe reduction

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U2 - 10.1016/j.watres.2017.04.044

DO - 10.1016/j.watres.2017.04.044

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SN - 0043-1354

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