Reducing RIP140 expression in macrophage alters ATM infiltration, facilitates white adipose tissue browning, and prevents high-fat diet-induced insulin resistance

Pu Ste Liu, Yi Wei Lin, Bomi Lee, Shelly K. McCrady-Spitzer, James A. Levine, Li Na Wei

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Adipose tissue macrophage (ATM) recruitment and activation play a critical role in obesity-induced inflammation and insulin resistance (IR). The mechanism regulating ATM activation and infiltration remains unclear. In this study, we found receptor interacting protein 140 (RIP140) can regulate the dynamics of ATM that contribute to adipose tissue remodeling. A high-fat diet (HFD) elevates RIP140 expression in macrophages. We generated mice with RIP140 knockdown in macrophages using transgenic and bone marrow transplantation procedures to blunt HFD-induced elevation in RIP140. We detected significant white adipose tissue (WAT) browning and improved systemic insulin sensitivity in these mice, particularly under an HFD feeding. These mice have decreased circulating monocyte population and altered ATM profile in WAT (a dramatic reduction in inflammatory classically activated macrophages [M1] and expansion in alternatively activated macrophages [M2]), which could improve HFD-induced IR. These studies suggest that reducing RIP140 expression in monocytes/macrophages can be a new therapeutic strategy in treating HFD-induced and inflammation-related diseases.

Original languageEnglish (US)
Pages (from-to)4021-4031
Number of pages11
JournalDiabetes
Volume63
Issue number12
DOIs
StatePublished - Dec 1 2014
Externally publishedYes

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Nuclear Receptor Co-Repressor 1
White Adipose Tissue
High Fat Diet
Insulin Resistance
Adipose Tissue
Macrophages
Macrophage Activation
Monocytes
Inflammation
Bone Marrow Transplantation
Obesity

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Reducing RIP140 expression in macrophage alters ATM infiltration, facilitates white adipose tissue browning, and prevents high-fat diet-induced insulin resistance. / Liu, Pu Ste; Lin, Yi Wei; Lee, Bomi; McCrady-Spitzer, Shelly K.; Levine, James A.; Wei, Li Na.

In: Diabetes, Vol. 63, No. 12, 01.12.2014, p. 4021-4031.

Research output: Contribution to journalArticle

Liu, Pu Ste ; Lin, Yi Wei ; Lee, Bomi ; McCrady-Spitzer, Shelly K. ; Levine, James A. ; Wei, Li Na. / Reducing RIP140 expression in macrophage alters ATM infiltration, facilitates white adipose tissue browning, and prevents high-fat diet-induced insulin resistance. In: Diabetes. 2014 ; Vol. 63, No. 12. pp. 4021-4031.
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