Reduced in vitro immune response on titania nanotube arrays compared to titanium surface

Barbara S. Smith, Patricia Capellato, Sean Kelley, Mercedes Gonzalez-Juarrero, Ketul C. Popat

Research output: Contribution to journalArticlepeer-review

64 Scopus citations


Material surfaces that provide biomimetic cues, such as nanoscale architectures, have been shown to alter cell/biomaterial interactions. Recent studies have identified titania nanotube arrays as strong candidates for use in interfaces on implantable devices due to their ability to elicit improved cellular functionality. However, limited information exists regarding the immune response of nanotube arrays. Thus, in this study, we have investigated the short- and long-term immune cell reaction of titania nanotube arrays. Whole blood lysate (containing leukocytes, thrombocytes and trace amounts of erythrocytes), isolated from human blood, were cultured on titania nanotube arrays and biomedical grade titanium (as a control) for 2 hours and 2 and 7 days. In order to determine the in vitro immune response on titania nanotube arrays, immune cell functionality was evaluated by cellular viability, adhesion, proliferation, morphology, cytokine/chemokine expression, with and without lipopolysaccharide (LPS), and nitric oxide release. The results presented in this study indicate a decrease in short- and long-term monocyte, macrophage and neutrophil functionality on titania nanotube arrays as compared to the control substrate. This work shows a reduced stimulation of the immune response on titania nanotube arrays, identifying this specific nanoarchitecture as a potentially optimal interface for implantable biomedical devices.

Original languageEnglish (US)
Pages (from-to)322-332
Number of pages11
JournalBiomaterials Science
Issue number3
StatePublished - Mar 1 2013
Externally publishedYes

ASJC Scopus subject areas

  • Biomedical Engineering
  • Materials Science(all)


Dive into the research topics of 'Reduced in vitro immune response on titania nanotube arrays compared to titanium surface'. Together they form a unique fingerprint.

Cite this