Reduced in vitro immune response on titania nanotube arrays compared to titanium surface

Barbara Smith, Patricia Capellato, Sean Kelley, Mercedes Gonzalez-Juarrero, Ketul C. Popat

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Material surfaces that provide biomimetic cues, such as nanoscale architectures, have been shown to alter cell/biomaterial interactions. Recent studies have identified titania nanotube arrays as strong candidates for use in interfaces on implantable devices due to their ability to elicit improved cellular functionality. However, limited information exists regarding the immune response of nanotube arrays. Thus, in this study, we have investigated the short- and long-term immune cell reaction of titania nanotube arrays. Whole blood lysate (containing leukocytes, thrombocytes and trace amounts of erythrocytes), isolated from human blood, were cultured on titania nanotube arrays and biomedical grade titanium (as a control) for 2 hours and 2 and 7 days. In order to determine the in vitro immune response on titania nanotube arrays, immune cell functionality was evaluated by cellular viability, adhesion, proliferation, morphology, cytokine/chemokine expression, with and without lipopolysaccharide (LPS), and nitric oxide release. The results presented in this study indicate a decrease in short- and long-term monocyte, macrophage and neutrophil functionality on titania nanotube arrays as compared to the control substrate. This work shows a reduced stimulation of the immune response on titania nanotube arrays, identifying this specific nanoarchitecture as a potentially optimal interface for implantable biomedical devices.

Original languageEnglish (US)
Pages (from-to)322-332
Number of pages11
JournalBiomaterials Science
Volume1
Issue number3
DOIs
StatePublished - Mar 2013
Externally publishedYes

Fingerprint

Titanium
Nanotubes
Blood
Macrophages
Nitric oxide
Biomimetics
Biocompatible Materials
titanium dioxide
Chemokines
Biomaterials
Lipopolysaccharides
Nitric Oxide
Adhesion
Cytokines
Substrates

ASJC Scopus subject areas

  • Materials Science(all)
  • Biomedical Engineering

Cite this

Reduced in vitro immune response on titania nanotube arrays compared to titanium surface. / Smith, Barbara; Capellato, Patricia; Kelley, Sean; Gonzalez-Juarrero, Mercedes; Popat, Ketul C.

In: Biomaterials Science, Vol. 1, No. 3, 03.2013, p. 322-332.

Research output: Contribution to journalArticle

Smith, B, Capellato, P, Kelley, S, Gonzalez-Juarrero, M & Popat, KC 2013, 'Reduced in vitro immune response on titania nanotube arrays compared to titanium surface', Biomaterials Science, vol. 1, no. 3, pp. 322-332. https://doi.org/10.1039/c2bm00079b
Smith, Barbara ; Capellato, Patricia ; Kelley, Sean ; Gonzalez-Juarrero, Mercedes ; Popat, Ketul C. / Reduced in vitro immune response on titania nanotube arrays compared to titanium surface. In: Biomaterials Science. 2013 ; Vol. 1, No. 3. pp. 322-332.
@article{fe5621dac57045f194e0547321bb999f,
title = "Reduced in vitro immune response on titania nanotube arrays compared to titanium surface",
abstract = "Material surfaces that provide biomimetic cues, such as nanoscale architectures, have been shown to alter cell/biomaterial interactions. Recent studies have identified titania nanotube arrays as strong candidates for use in interfaces on implantable devices due to their ability to elicit improved cellular functionality. However, limited information exists regarding the immune response of nanotube arrays. Thus, in this study, we have investigated the short- and long-term immune cell reaction of titania nanotube arrays. Whole blood lysate (containing leukocytes, thrombocytes and trace amounts of erythrocytes), isolated from human blood, were cultured on titania nanotube arrays and biomedical grade titanium (as a control) for 2 hours and 2 and 7 days. In order to determine the in vitro immune response on titania nanotube arrays, immune cell functionality was evaluated by cellular viability, adhesion, proliferation, morphology, cytokine/chemokine expression, with and without lipopolysaccharide (LPS), and nitric oxide release. The results presented in this study indicate a decrease in short- and long-term monocyte, macrophage and neutrophil functionality on titania nanotube arrays as compared to the control substrate. This work shows a reduced stimulation of the immune response on titania nanotube arrays, identifying this specific nanoarchitecture as a potentially optimal interface for implantable biomedical devices.",
author = "Barbara Smith and Patricia Capellato and Sean Kelley and Mercedes Gonzalez-Juarrero and Popat, {Ketul C.}",
year = "2013",
month = "3",
doi = "10.1039/c2bm00079b",
language = "English (US)",
volume = "1",
pages = "322--332",
journal = "Biomaterials Science",
issn = "2047-4830",
publisher = "Royal Society of Chemistry",
number = "3",

}

TY - JOUR

T1 - Reduced in vitro immune response on titania nanotube arrays compared to titanium surface

AU - Smith, Barbara

AU - Capellato, Patricia

AU - Kelley, Sean

AU - Gonzalez-Juarrero, Mercedes

AU - Popat, Ketul C.

PY - 2013/3

Y1 - 2013/3

N2 - Material surfaces that provide biomimetic cues, such as nanoscale architectures, have been shown to alter cell/biomaterial interactions. Recent studies have identified titania nanotube arrays as strong candidates for use in interfaces on implantable devices due to their ability to elicit improved cellular functionality. However, limited information exists regarding the immune response of nanotube arrays. Thus, in this study, we have investigated the short- and long-term immune cell reaction of titania nanotube arrays. Whole blood lysate (containing leukocytes, thrombocytes and trace amounts of erythrocytes), isolated from human blood, were cultured on titania nanotube arrays and biomedical grade titanium (as a control) for 2 hours and 2 and 7 days. In order to determine the in vitro immune response on titania nanotube arrays, immune cell functionality was evaluated by cellular viability, adhesion, proliferation, morphology, cytokine/chemokine expression, with and without lipopolysaccharide (LPS), and nitric oxide release. The results presented in this study indicate a decrease in short- and long-term monocyte, macrophage and neutrophil functionality on titania nanotube arrays as compared to the control substrate. This work shows a reduced stimulation of the immune response on titania nanotube arrays, identifying this specific nanoarchitecture as a potentially optimal interface for implantable biomedical devices.

AB - Material surfaces that provide biomimetic cues, such as nanoscale architectures, have been shown to alter cell/biomaterial interactions. Recent studies have identified titania nanotube arrays as strong candidates for use in interfaces on implantable devices due to their ability to elicit improved cellular functionality. However, limited information exists regarding the immune response of nanotube arrays. Thus, in this study, we have investigated the short- and long-term immune cell reaction of titania nanotube arrays. Whole blood lysate (containing leukocytes, thrombocytes and trace amounts of erythrocytes), isolated from human blood, were cultured on titania nanotube arrays and biomedical grade titanium (as a control) for 2 hours and 2 and 7 days. In order to determine the in vitro immune response on titania nanotube arrays, immune cell functionality was evaluated by cellular viability, adhesion, proliferation, morphology, cytokine/chemokine expression, with and without lipopolysaccharide (LPS), and nitric oxide release. The results presented in this study indicate a decrease in short- and long-term monocyte, macrophage and neutrophil functionality on titania nanotube arrays as compared to the control substrate. This work shows a reduced stimulation of the immune response on titania nanotube arrays, identifying this specific nanoarchitecture as a potentially optimal interface for implantable biomedical devices.

UR - http://www.scopus.com/inward/record.url?scp=84885332118&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84885332118&partnerID=8YFLogxK

U2 - 10.1039/c2bm00079b

DO - 10.1039/c2bm00079b

M3 - Article

VL - 1

SP - 322

EP - 332

JO - Biomaterials Science

JF - Biomaterials Science

SN - 2047-4830

IS - 3

ER -