Redox-sensitive homodimerization of Pex11p

A proposed mechanism to regulate peroxisomal division

Pamela Marshall, John M. Dyer, Mary E. Quick, Joel M. Goodman

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Pex11p (formerly Pmp27) has been implicated in peroxisomal proliferation (Erdmann, R., and G. Blobel, 1995, J. Cell Biol. 128: 509-523; Marshall, P.A., Y.I. Krimkevich, R.H. Lark, J.M. Dyer, M. Veenhuis, and J.M. Goodman, 1995, J. Cell Biol. 129; 345-355). In its absence, peroxisomes in Saccharomyces cerevisiae fail to proliferate in response to oleic acid; instead, one or two large peroxisomes are formed. Conversely, overproduction of Pex11p causes an increase in peroxisomal number. In this report, we confirm the function of Pex11p in organelle proliferation by demonstrating that this protein can cause fragmentation in vivo of large peroxisomes into smaller organelles. Pex11p is on the inner surface of the peroxisomal membrane. It can form homodimers, and this species is more abundant in mature peroxisomes than in species is more abundant in mature peroxisomes than in proliferating organelles. Removing one of the three cysteines in the protein inhibits homodimerization. This cysteine 3 → alanine mutation leads to an increase in number and a decrease in peroxisomal density, compared with the wild-type protein, in response to oleic acid. We propose that the active species is the 'monomeric' form, and that the increasing oxidative metabolism within maturing peroxisomes causes dimer formation and inhibition of further organelle division.

Original languageEnglish (US)
Pages (from-to)123-137
Number of pages15
JournalJournal of Cell Biology
Volume135
Issue number1
DOIs
StatePublished - Oct 1996
Externally publishedYes

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Peroxisomes
Oxidation-Reduction
Organelles
Oleic Acid
Cysteine
Proteins
Alanine
Saccharomyces cerevisiae
Mutation
Membranes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Redox-sensitive homodimerization of Pex11p : A proposed mechanism to regulate peroxisomal division. / Marshall, Pamela; Dyer, John M.; Quick, Mary E.; Goodman, Joel M.

In: Journal of Cell Biology, Vol. 135, No. 1, 10.1996, p. 123-137.

Research output: Contribution to journalArticle

Marshall, Pamela ; Dyer, John M. ; Quick, Mary E. ; Goodman, Joel M. / Redox-sensitive homodimerization of Pex11p : A proposed mechanism to regulate peroxisomal division. In: Journal of Cell Biology. 1996 ; Vol. 135, No. 1. pp. 123-137.
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