Red antenna states of photosystem i from cyanobacteria Synechocystis PCC 6803 and Thermosynechococcus elongatus: Single-complex spectroscopy and spectral hole-burning study

Kerry J. Riley, Tõnu Reinot, Ryszard Jankowiak, Petra Fromme, Valter Zazubovich

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Abstract

Hole-burning and single photosynthetic complex spectroscopy were used to study the excitonic structure and excitation energy-transfer processes of cyanobacterial trimeric Photosystem I (PS I) complexes from Synechocystis PCC 6803 and Thermosynechococcus elongatus at low temperatures. It was shown that individual PS I complexes of Synechocystis PCC 6803 (which have two red antenna states, i.e., C706 and C714) reveal only a broad structureless fluorescence band with a maximum near 720 nm, indicating strong electron-phonon coupling for the lowest energy C714 red state. The absence of zero-phonon lines (ZPLs) belonging to the C706 red state in the emission spectra of individual PS I complexes from Synechocystis PCC 6803 suggests that the C706 and C714 red antenna states of Synechocystis PCC 6803 are connected by efficient energy transfer with a characteristic transfer time of ∼5 ps. This finding is in agreement with spectral hole-burning data obtained for bulk samples of Synechocystis PCC 6803. The importance of comparing the results of ensemble (spectral hole burning) and single-complex measurements was demonstrated. The presence of narrow ZPLs near 710 nm in addition to the broad fluorescence band at ∼730 nm in Thermosynechococcus elongatus (Jelezko et al. J. Phys. Chem. B 2000, 104, 8093-8096) has been confirmed. We also demonstrate that high-quality samples obtained by dissolving crystals of PS I of Thermosynechococcus elongatus exhibit stronger absorption in the red antenna region than any samples studied so far by us and other groups.

Original languageEnglish (US)
Pages (from-to)286-292
Number of pages7
JournalJournal of Physical Chemistry B
Volume111
Issue number1
DOIs
StatePublished - Jan 11 2007

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Photosystem I Protein Complex
hole burning
antennas
Spectroscopy
Antennas
Energy transfer
Fluorescence
energy transfer
spectroscopy
fluorescence
Excitation energy
Photosynthetic Reaction Center Complex Proteins
dissolving
emission spectra
Crystals
Electrons
excitation
crystals
Cyanobacteria
electrons

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Cite this

Red antenna states of photosystem i from cyanobacteria Synechocystis PCC 6803 and Thermosynechococcus elongatus : Single-complex spectroscopy and spectral hole-burning study. / Riley, Kerry J.; Reinot, Tõnu; Jankowiak, Ryszard; Fromme, Petra; Zazubovich, Valter.

In: Journal of Physical Chemistry B, Vol. 111, No. 1, 11.01.2007, p. 286-292.

Research output: Contribution to journalArticle

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abstract = "Hole-burning and single photosynthetic complex spectroscopy were used to study the excitonic structure and excitation energy-transfer processes of cyanobacterial trimeric Photosystem I (PS I) complexes from Synechocystis PCC 6803 and Thermosynechococcus elongatus at low temperatures. It was shown that individual PS I complexes of Synechocystis PCC 6803 (which have two red antenna states, i.e., C706 and C714) reveal only a broad structureless fluorescence band with a maximum near 720 nm, indicating strong electron-phonon coupling for the lowest energy C714 red state. The absence of zero-phonon lines (ZPLs) belonging to the C706 red state in the emission spectra of individual PS I complexes from Synechocystis PCC 6803 suggests that the C706 and C714 red antenna states of Synechocystis PCC 6803 are connected by efficient energy transfer with a characteristic transfer time of ∼5 ps. This finding is in agreement with spectral hole-burning data obtained for bulk samples of Synechocystis PCC 6803. The importance of comparing the results of ensemble (spectral hole burning) and single-complex measurements was demonstrated. The presence of narrow ZPLs near 710 nm in addition to the broad fluorescence band at ∼730 nm in Thermosynechococcus elongatus (Jelezko et al. J. Phys. Chem. B 2000, 104, 8093-8096) has been confirmed. We also demonstrate that high-quality samples obtained by dissolving crystals of PS I of Thermosynechococcus elongatus exhibit stronger absorption in the red antenna region than any samples studied so far by us and other groups.",
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