TY - JOUR
T1 - Recovery of an oligonucleotide using silver ions immobilized onto paramagnetic particles
AU - Ramírez-Vick, Jaime E.
AU - Garcia, Antonio
AU - Lee, James
N1 - Funding Information:
The authors would like to thank the National Science Foundation for support for this research (BES-9521668).
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1998
Y1 - 1998
N2 - A 3'-GCCCCTTTTTAAAAACCCCG-5' oligonucleotide can be recovered from aqueous solution using paramagnetic particles containing immobilized silver ions. Binding and elution experiments were conducted by attaching either fluorescein isothioocyanate (FITC) to the 3'-end or Texas Red or fluorescein phosphoramidite (FAM) to the 5'-end of the oligonucleotide. For the 5'-end FAM labeled oligonucleotide, a binding constant of 4.2 x 107 was measured at pH 7 using phosphate buffer. The percent of bound FAM labeled oligonucleotide eluted from the paramagnetic particles was found to be 97% using an aqueous solution of thiodiglycol. While the FAM molecule by itself does not bind to the silver activated paramagnetic particles, the choice of fluorescent label affects binding affinities and elution recoveries.
AB - A 3'-GCCCCTTTTTAAAAACCCCG-5' oligonucleotide can be recovered from aqueous solution using paramagnetic particles containing immobilized silver ions. Binding and elution experiments were conducted by attaching either fluorescein isothioocyanate (FITC) to the 3'-end or Texas Red or fluorescein phosphoramidite (FAM) to the 5'-end of the oligonucleotide. For the 5'-end FAM labeled oligonucleotide, a binding constant of 4.2 x 107 was measured at pH 7 using phosphate buffer. The percent of bound FAM labeled oligonucleotide eluted from the paramagnetic particles was found to be 97% using an aqueous solution of thiodiglycol. While the FAM molecule by itself does not bind to the silver activated paramagnetic particles, the choice of fluorescent label affects binding affinities and elution recoveries.
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U2 - 10.1080/10826069808010139
DO - 10.1080/10826069808010139
M3 - Article
C2 - 9710897
AN - SCOPUS:0032146190
VL - 28
SP - 243
EP - 260
JO - Preparative Biochemistry and Biotechnology
JF - Preparative Biochemistry and Biotechnology
SN - 1082-6068
IS - 3
ER -