Reconstitution of the Gs protein from B16 melanoma clones of high and low experimental metastatic potential into S49 cyc- membranes

Bruce R. Lester, Jeffrey M. Stadel, Charles Buscarino, John Sheppard, Russell G. Greig, George Poste

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The ability of a series of B16 melanoma clones to form experimental lung metastases in syngeneic mice has been shown to correlate positively with adenylate cyclase activity. (Sheppard et al, Int. J. Cancer 37 (1986) 713-722). To begin to identify the components of the adenylate cyclase complex that account for enhanced enzyme activity in highly metastatic tumor populations, cholate extracts containing the GTP-binding protein Gs from B16 melanoma clones of different metastatic capacities were reconstituted with membranes prepared from S49 cyc-, a variant lymphoma cell line that lacks Gs function. The results revealed that extracts from a highly metastatic B16 clone (F10-C23) reconstituted significantly greater adenylate cyclase activities in S49 cyc- membranes than parallel preparations from a B16 clone (F1-C29) of low metastatic capacity. The data suggest that aberrations in Gs function may contribute to the heightened responsiveness of adenylate cyclase observed in B16 melanoma clones of increased metastatic potential.

Original languageEnglish (US)
Pages (from-to)443-451
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume147
Issue number1
DOIs
StatePublished - Aug 31 1987
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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