Recombinational cloning.

Jaehong Park, Joshua LaBaer

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The study of protein en masse, or functional proteomics, depends on the availability of full-length cDNAs in appropriate expression-ready plasmid vectors for protein expression and functional analysis. Recombinational cloning is a universal cloning technique based on site-specific recombination that is independent of the insert DNA sequence to be cloned, which differentiates this method from the classical restriction enzyme-based cloning methods. Recombinational cloning enables rapid and efficient parallel transfer of DNA inserts into multiple expression systems. This unit summarizes strategies for generating expression-ready clones using two of the most popular recombinational cloning technologies, now commercially available from Invitrogen (Gateway) and BD Clontech (Creator).

Original languageEnglish (US)
JournalCurrent protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]
VolumeChapter 3
StatePublished - May 2006
Externally publishedYes

Fingerprint

Organism Cloning
Proteomics
Genetic Recombination
Proteins
Plasmids
Complementary DNA
Clone Cells
Technology
DNA
Enzymes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Recombinational cloning. / Park, Jaehong; LaBaer, Joshua.

In: Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.], Vol. Chapter 3, 05.2006.

Research output: Contribution to journalArticle

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