Recombinant fowlpox viruses coexpressing chicken type I IFN and newcastle disease virus HN and F genes: Influence of IFN on protective efficacy and humoral responses of chickens following in ovo or post-hatch administration of recombinant viruses

Kemal Karaca, Jagdev M. Sharma, Barbara J. Winslow, David E. Junker, Sudhir Reddy, Mark Cochran, Janis McMillen

Research output: Contribution to journalArticle

68 Scopus citations

Abstract

We have constructed recombinant (r) fowl pox viruses (FPVs) coexpressing chicken type I interferon (IFN) and/or hemagglutinin-neuraminidase (HN) and fusion (F) proteins of Newcastle disease virus (NDV). We administered rFPVs and FPV into embryonated chicken eggs at 17 days of embryonation or in chickens after hatch. Administration of FPV or rFPVs did not influence hatchability and survival of hatched chicks. In ovo or after hatch vaccination of chickens with the recombinant viruses resulted in protection against challenge with virulent FPV and NDL7. Chickens vaccinated with FPV or FPV-NDV recombinant had significantly lower body weight 2 weeks following vaccination. This loss in body weight was not detected in chickens receiving FPV-IFN and FPV-NDV-IFN recombinants. Chickens vaccinated with FPV coexpressing IFN and NDV genes produced less antibodies against NDV in comparison with chickens vaccinated with FPV expressing NDV genes.

Original languageEnglish (US)
Pages (from-to)1496-1503
Number of pages8
JournalVaccine
Volume16
Issue number16
DOIs
StatePublished - Oct 1 1998

Keywords

  • Chicken
  • Hemagglutinin- neuraminidase of Newcastle disease virus
  • In ovo
  • Recombinant fowlpox virus
  • Type I interferon

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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