Recombinant Coccidioides immitis complement-fixing antigen: Detection of an epitope shared by C. immitis, Histoplasma capsulatum, and Blastomyces dermatitidis

Michael Chunmu Yang, Dewey Magee, Leo Kaufman, Yufan Zhu, Rebecca A. Cox

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We undertook an investigation to assess the utility of a recombinant Coccidioides immitis complement-fixing (CF) antigen for detecting CF antibody in sera from patients with coccidioidomycosis. Enzyme-linked immunosorbent assays established that recombinant CF antigen and, for comparison, a commercially available coccidioidin were reactive with 19 of 19 sera from patients with active coccidioidomycosis. The recombinant antigen was significantly more sensitive than coccidioidin. The median titer obtained when patients' sera were assayed against recombinant CF antigen was 1:51,200 compared to 1:25,600 with coccidioidin (P < 0.027). The recombinant antigen was also more effective in distinguishing the antibody levels in sera from patients with pulmonary coccidioidomycosis than in sera from those with disseminated disease. Whereas patients with pulmonary disease showed a median antibody titer of 1:25,600, those with multifocal disease showed a median titer of 1:102,400 (P < 0.028). The recombinant CF antigen was found, however, to express an epitope(s) that reacted with sera from 6 of 12 patients with histoplasmosis and 2 of 12 patients with blastomycosis.

Original languageEnglish (US)
Pages (from-to)19-22
Number of pages4
JournalClinical and Diagnostic Laboratory Immunology
Volume4
Issue number1
StatePublished - 1997
Externally publishedYes

Fingerprint

Blastomyces
Coccidioides
Histoplasma
Epitopes
Coccidioidin
Antigens
Coccidioidomycosis
Serum
Antibodies
Immunosorbents
Pulmonary diseases
Blastomycosis
Histoplasmosis
Assays
Lung Diseases
Enzyme-Linked Immunosorbent Assay
Lung
Enzymes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology and Allergy
  • Clinical Biochemistry
  • Immunology

Cite this

Recombinant Coccidioides immitis complement-fixing antigen : Detection of an epitope shared by C. immitis, Histoplasma capsulatum, and Blastomyces dermatitidis. / Yang, Michael Chunmu; Magee, Dewey; Kaufman, Leo; Zhu, Yufan; Cox, Rebecca A.

In: Clinical and Diagnostic Laboratory Immunology, Vol. 4, No. 1, 1997, p. 19-22.

Research output: Contribution to journalArticle

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