Abstract
Anti-carbohydrate antibodies were isolated from Human immunodeficiency virus (HIV) negative human serum by affinity chromatography using yeast mannan followed by protein A. The purified mannan-binding IgG (MBIgG) bound to HIV glycoproteins gp160, gp120 and gp41 in Western blot. Immunofluorescence revealed that MBIgG bound to HIV IIIB-infected H9 cells but not to uninfected H9 cells, suggesting that carbohydrate structures recognized by MBIgG are specifically expressed on HIV-infected cells. MBIgG did not neutralize infectivity of HIV. These results show that normal human serum contains natural antibodies reactive to carbohydrate structures of HIV glycoproteins propagated in human cells.
Original language | English (US) |
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Pages (from-to) | 279-285 |
Number of pages | 7 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 177 |
Issue number | 1 |
DOIs | |
State | Published - May 31 1991 |
Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology