Recognition of human immunodeficiency virus glycoproteins by natural anti-carbohydrate antibodies in human serum

Takami Tomiyama, Douglas Lake, Yasuhiko Masuho, Evan M. Hersh

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Anti-carbohydrate antibodies were isolated from Human immunodeficiency virus (HIV) negative human serum by affinity chromatography using yeast mannan followed by protein A. The purified mannan-binding IgG (MBIgG) bound to HIV glycoproteins gp160, gp120 and gp41 in Western blot. Immunofluorescence revealed that MBIgG bound to HIV IIIB-infected H9 cells but not to uninfected H9 cells, suggesting that carbohydrate structures recognized by MBIgG are specifically expressed on HIV-infected cells. MBIgG did not neutralize infectivity of HIV. These results show that normal human serum contains natural antibodies reactive to carbohydrate structures of HIV glycoproteins propagated in human cells.

Original languageEnglish (US)
Pages (from-to)279-285
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume177
Issue number1
DOIs
StatePublished - May 31 1991
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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