Recognition of cysteine-containing peptides through prompt fragmentation of the 4-dimethylaminophenylazophenyl-4′-maleimide derivative during analysis by MALDI-MS

Chad R. Borges, J. Throck Watson

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Under specified UV-MALDI conditions, the 4-dimethylaminophenylazophenyl-4′-maleimide (DABMI) derivative of cysteine-containing peptides undergoes prompt fragmentation to produce a characteristic mass spectral pattern. As reported previously by others, derivatization with chromophoric DABMI allows cysteine-containing peptides and proteins to be tracked during HPLC by absorbance at upper UV and visible wavelengths. Here, we describe methodology by which these same peptide derivatives can be recognized by their distinctive MALDI mass spectral fragmentation pattern, then mass mapped, allowing for easy, rapid identification of cysteine-containing peptides.

Original languageEnglish (US)
Pages (from-to)1567-1572
Number of pages6
JournalProtein Science
Volume12
Issue number7
DOIs
StatePublished - Jul 1 2003

Keywords

  • DABMI
  • MALDI
  • Prompt fragmentation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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