Recognition of cysteine-containing peptides through prompt fragmentation of the 4-dimethylaminophenylazophenyl-4′-maleimide derivative during analysis by MALDI-MS

Chad Borges, J. Throck Watson

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Under specified UV-MALDI conditions, the 4-dimethylaminophenylazophenyl-4′-maleimide (DABMI) derivative of cysteine-containing peptides undergoes prompt fragmentation to produce a characteristic mass spectral pattern. As reported previously by others, derivatization with chromophoric DABMI allows cysteine-containing peptides and proteins to be tracked during HPLC by absorbance at upper UV and visible wavelengths. Here, we describe methodology by which these same peptide derivatives can be recognized by their distinctive MALDI mass spectral fragmentation pattern, then mass mapped, allowing for easy, rapid identification of cysteine-containing peptides.

Original languageEnglish (US)
Pages (from-to)1567-1572
Number of pages6
JournalProtein Science
Volume12
Issue number7
DOIs
StatePublished - Jul 1 2003
Externally publishedYes

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Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Cysteine
Derivatives
Peptides
High Pressure Liquid Chromatography
Wavelength
4-dimethylaminophenylazophenyl-4'-maleimide
Proteins

Keywords

  • DABMI
  • MALDI
  • Prompt fragmentation

ASJC Scopus subject areas

  • Biochemistry

Cite this

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abstract = "Under specified UV-MALDI conditions, the 4-dimethylaminophenylazophenyl-4′-maleimide (DABMI) derivative of cysteine-containing peptides undergoes prompt fragmentation to produce a characteristic mass spectral pattern. As reported previously by others, derivatization with chromophoric DABMI allows cysteine-containing peptides and proteins to be tracked during HPLC by absorbance at upper UV and visible wavelengths. Here, we describe methodology by which these same peptide derivatives can be recognized by their distinctive MALDI mass spectral fragmentation pattern, then mass mapped, allowing for easy, rapid identification of cysteine-containing peptides.",
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AU - Watson, J. Throck

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AB - Under specified UV-MALDI conditions, the 4-dimethylaminophenylazophenyl-4′-maleimide (DABMI) derivative of cysteine-containing peptides undergoes prompt fragmentation to produce a characteristic mass spectral pattern. As reported previously by others, derivatization with chromophoric DABMI allows cysteine-containing peptides and proteins to be tracked during HPLC by absorbance at upper UV and visible wavelengths. Here, we describe methodology by which these same peptide derivatives can be recognized by their distinctive MALDI mass spectral fragmentation pattern, then mass mapped, allowing for easy, rapid identification of cysteine-containing peptides.

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