Recognition imaging with a DNA aptamer

Liyun Lin; Hongda Wang; Yan Liu; Hao Yan; Stuart Lindsay

doi:10.1529/biophysj.105.079111

Recognition imaging with a DNA aptamer

Liyun Lin, Hongda Wang, Yan Liu, Hao Yan, Stuart Lindsay

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

We have used a DNA-aptamer tethered to an atomic force microscope probe to carry out recognition imaging of IgE molecules attached to a mica substrate. The recognition was efficient (∼90%) and specific, being blocked by injection of IgE molecules in solution, and not being interfered with by high concentrations of a second protein. The signal/noise ratio of the recognition signal was better than that obtained with antibodies, despite the fact that the average force required to break the aptamer-protein bonds was somewhat smaller.

Original language	English (US)
Pages (from-to)	4236-4238
Number of pages	3
Journal	Biophysical journal
Volume	90
Issue number	11
DOIs	https://doi.org/10.1529/biophysj.105.079111
State	Published - Jun 2006

ASJC Scopus subject areas

Biophysics

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title = "Recognition imaging with a DNA aptamer",

abstract = "We have used a DNA-aptamer tethered to an atomic force microscope probe to carry out recognition imaging of IgE molecules attached to a mica substrate. The recognition was efficient (∼90%) and specific, being blocked by injection of IgE molecules in solution, and not being interfered with by high concentrations of a second protein. The signal/noise ratio of the recognition signal was better than that obtained with antibodies, despite the fact that the average force required to break the aptamer-protein bonds was somewhat smaller.",

author = "Liyun Lin and Hongda Wang and Yan Liu and Hao Yan and Stuart Lindsay",

note = "Funding Information: This work was supported by National Institutes of Health (CA 85990) and the National Science Foundation (CCF-0453686, CCF-0453685).",

year = "2006",

month = jun,

doi = "10.1529/biophysj.105.079111",

language = "English (US)",

volume = "90",

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journal = "Biophysical journal",

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T1 - Recognition imaging with a DNA aptamer

AU - Lin, Liyun

AU - Wang, Hongda

AU - Liu, Yan

AU - Yan, Hao

AU - Lindsay, Stuart

N1 - Funding Information: This work was supported by National Institutes of Health (CA 85990) and the National Science Foundation (CCF-0453686, CCF-0453685).

PY - 2006/6

Y1 - 2006/6

N2 - We have used a DNA-aptamer tethered to an atomic force microscope probe to carry out recognition imaging of IgE molecules attached to a mica substrate. The recognition was efficient (∼90%) and specific, being blocked by injection of IgE molecules in solution, and not being interfered with by high concentrations of a second protein. The signal/noise ratio of the recognition signal was better than that obtained with antibodies, despite the fact that the average force required to break the aptamer-protein bonds was somewhat smaller.

AB - We have used a DNA-aptamer tethered to an atomic force microscope probe to carry out recognition imaging of IgE molecules attached to a mica substrate. The recognition was efficient (∼90%) and specific, being blocked by injection of IgE molecules in solution, and not being interfered with by high concentrations of a second protein. The signal/noise ratio of the recognition signal was better than that obtained with antibodies, despite the fact that the average force required to break the aptamer-protein bonds was somewhat smaller.

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DO - 10.1529/biophysj.105.079111

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SN - 0006-3495

VL - 90

SP - 4236

EP - 4238

JO - Biophysical journal

JF - Biophysical journal

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ER -

Recognition imaging with a DNA aptamer

Abstract

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