Recellularization of decellularized lung scaffolds is enhanced by dynamic suspension culture

Aurélie Crabbé, Yulong Liu, Shameema F. Sarker, Nicholas R. Bonenfant, Jennifer Barrila, Zachary D. Borg, James J. Lee, Daniel J. Weiss, Cheryl Nickerson

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Strategies are needed to improve repopulation of decellularized lung scaffolds with stromal and functional epithelial cells. We demonstrate that decellularized mouse lungs recellularized in a dynamic low fluid shear suspension bioreactor, termed the rotating wall vessel (RWV), contained more cells with decreased apoptosis, increased proliferation and enhanced levels of total RNA compared to static recellularization conditions. These results were observed with two relevant mouse cell types: bone marrow-derived mesenchymal stromal (stem) cells (MSCs) and alveolar type II cells (C10). In addition, MSCs cultured in decellularized lungs under static but not bioreactor conditions formed multilayered aggregates. Gene expression and immunohistochemical analyses suggested differentiation of MSCs into collagen I-producing fibroblast-like cells in the bioreactor, indicating enhanced potential for remodeling of the decellularized scaffold matrix. In conclusion, dynamic suspension culture is promising for enhancing repopulation of decellularized lungs, and could contribute to remodeling the extracellular matrix of the scaffolds with subsequent effects on differentiation and functionality of inoculated cells.

Original languageEnglish (US)
Article numbere0126846
JournalPLoS One
Volume10
Issue number5
DOIs
StatePublished - May 11 2015

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Bioreactors
Scaffolds
Suspensions
lungs
Lung
bioreactors
Mesenchymal Stromal Cells
cells
Fibroblasts
Stem cells
Alveolar Epithelial Cells
Gene expression
Bone
Collagen
Hydrodynamics
Bone Marrow Cells
RNA
Apoptosis
stromal cells
Extracellular Matrix

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Recellularization of decellularized lung scaffolds is enhanced by dynamic suspension culture. / Crabbé, Aurélie; Liu, Yulong; Sarker, Shameema F.; Bonenfant, Nicholas R.; Barrila, Jennifer; Borg, Zachary D.; Lee, James J.; Weiss, Daniel J.; Nickerson, Cheryl.

In: PLoS One, Vol. 10, No. 5, e0126846, 11.05.2015.

Research output: Contribution to journalArticle

Crabbé, A, Liu, Y, Sarker, SF, Bonenfant, NR, Barrila, J, Borg, ZD, Lee, JJ, Weiss, DJ & Nickerson, C 2015, 'Recellularization of decellularized lung scaffolds is enhanced by dynamic suspension culture', PLoS One, vol. 10, no. 5, e0126846. https://doi.org/10.1371/journal.pone.0126846
Crabbé, Aurélie ; Liu, Yulong ; Sarker, Shameema F. ; Bonenfant, Nicholas R. ; Barrila, Jennifer ; Borg, Zachary D. ; Lee, James J. ; Weiss, Daniel J. ; Nickerson, Cheryl. / Recellularization of decellularized lung scaffolds is enhanced by dynamic suspension culture. In: PLoS One. 2015 ; Vol. 10, No. 5.
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