@article{d8164e8ba11e4110b10efbe55587e7f6,
title = "Recellularization of decellularized lung scaffolds is enhanced by dynamic suspension culture",
abstract = "Strategies are needed to improve repopulation of decellularized lung scaffolds with stromal and functional epithelial cells. We demonstrate that decellularized mouse lungs recellularized in a dynamic low fluid shear suspension bioreactor, termed the rotating wall vessel (RWV), contained more cells with decreased apoptosis, increased proliferation and enhanced levels of total RNA compared to static recellularization conditions. These results were observed with two relevant mouse cell types: bone marrow-derived mesenchymal stromal (stem) cells (MSCs) and alveolar type II cells (C10). In addition, MSCs cultured in decellularized lungs under static but not bioreactor conditions formed multilayered aggregates. Gene expression and immunohistochemical analyses suggested differentiation of MSCs into collagen I-producing fibroblast-like cells in the bioreactor, indicating enhanced potential for remodeling of the decellularized scaffold matrix. In conclusion, dynamic suspension culture is promising for enhancing repopulation of decellularized lungs, and could contribute to remodeling the extracellular matrix of the scaffolds with subsequent effects on differentiation and functionality of inoculated cells.",
author = "Aur{\'e}lie Crabb{\'e} and Yulong Liu and Sarker, {Shameema F.} and Bonenfant, {Nicholas R.} and Jennifer Barrila and Borg, {Zachary D.} and Lee, {James J.} and Weiss, {Daniel J.} and Cheryl Nickerson",
note = "Funding Information: We are grateful to Jenny Pattengill (Mayo Clinic Histology Core Facility) for processing samples for histology. We thank Phillip Stafford (The Biodesign Institute, Innovations in Medicine) for his expertise in bioinformatics data analysis for this work. We thank Mark Ott and his team at the Microbiology Laboratory at the NASA Johnson Space Center for their support of data analysis. We thank John Wallis for helpful suggestions on recellularizing decellularized lung scaffolds. This work was funded by the National Institutes of Health (NIH grants RC4HL106625 (DJW and CAN), NHLBI R21HL108689 (DJW), NHLBI R21HL094611 (DJW), NIGMS COBRE P30 GM103532 (C Irvin PI)), the National Aeronautics and Space Administration (NASA grants NNX10AO52G (CAN) and NNX13AM01G (CAN)) and by a Mayo Clinic-ASU Seed grant (AC, CAN, JJL). Publisher Copyright: {\textcopyright} 2015 Crabb{\'e} et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",
year = "2015",
month = may,
day = "11",
doi = "10.1371/journal.pone.0126846",
language = "English (US)",
volume = "10",
journal = "PloS one",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "5",
}