Real-time polymerase chain reaction quantification of canine DNA

Jeffrey J. Evans, Elizabeth J. Wictum, M. Cecilia T Penedo, Sreetharan Kanthaswamy

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The accurate quantification of target DNA is an important step in the short tandem repeat analysis of forensic biological samples. By utilizing quantification data to control the amount of template DNA in the polymerase chain reaction (PCR), forensic scientists can optimize testing and minimize the consumption of limited samples. The ability to identify and quantify target DNA in mixed-species samples is crucial when it may be overwhelmed by nontarget DNA, as in cases of dog attack. We evaluated two quantitative real-time PCR assays for dynamic range, species specificity, and inhibition by humic acid. While both assays proved to be highly sensitive and discriminating, the Melanocortin-1 Receptor (MC1R) gene Taqman® assay had the advantages of a shorter run time, greater efficiency, and safer reagents. In its application to forensic casework, the MC1R assay has been advantageous for quantifying dog DNA in a variety of mixed-species samples and facilitating the successful profiling of individual dogs.

Original languageEnglish (US)
Pages (from-to)93-96
Number of pages4
JournalJournal of Forensic Sciences
Volume52
Issue number1
DOIs
StatePublished - Jan 2007
Externally publishedYes

Fingerprint

quantification
Canidae
Real-Time Polymerase Chain Reaction
Receptor, Melanocortin, Type 1
DNA
Dogs
Humic Substances
Species Specificity
casework
DNA-Directed DNA Polymerase
Microsatellite Repeats
Polymerase Chain Reaction
efficiency
time
ability
Genes

Keywords

  • Canine
  • DNA quantification
  • Dog
  • Forensic science
  • MC1R
  • QPCR
  • Real-time PCR

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Law

Cite this

Real-time polymerase chain reaction quantification of canine DNA. / Evans, Jeffrey J.; Wictum, Elizabeth J.; Penedo, M. Cecilia T; Kanthaswamy, Sreetharan.

In: Journal of Forensic Sciences, Vol. 52, No. 1, 01.2007, p. 93-96.

Research output: Contribution to journalArticle

Evans, Jeffrey J. ; Wictum, Elizabeth J. ; Penedo, M. Cecilia T ; Kanthaswamy, Sreetharan. / Real-time polymerase chain reaction quantification of canine DNA. In: Journal of Forensic Sciences. 2007 ; Vol. 52, No. 1. pp. 93-96.
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