RcsB and RcsC: A two-component regulator of capsule synthesis in Escherichia coli

V. Stout, S. Gottesman

Research output: Contribution to journalArticle

173 Citations (Scopus)

Abstract

Colanic acid capsule synthesis in Escherichia coli K-12 is regulated by RcsB and RcsC. The amino acid sequences of these two proteins, deduced from the nucleotide sequence reported here, demonstrate their homology to environmentally responsive two-component regulators that have been reported in both gram-positive and gram-negative bacteria. In our model, RcsC acts as the sensor and RcsB acts as the receiver or effector to stimulate capsule synthesis from cps genes. In addition, RcsC shows limited homology to the other effectors in its C terminus. Fusions of rscC to phoA that resulted in PhoA+ strains demonstrated that RcsC is a transmembrane protein with a periplasmic N-terminal domain and cytoplasmic C-terminal domain. Additional control of this regulatory network is provided by the dependence on the alternate sigma factor, RpoN, for the synthesis of RcsB. The rcsB and rcsC genes, which are oriented convergently with their stop codons 196 base pairs apart, are separated by a long direct repeat including two repetitive extragenic palindromic sequences.

Original languageEnglish (US)
Pages (from-to)659-669
Number of pages11
JournalJournal of Bacteriology
Volume172
Issue number2
StatePublished - 1990
Externally publishedYes

Fingerprint

Capsules
Escherichia coli
Sigma Factor
Terminator Codon
Nucleic Acid Repetitive Sequences
Gram-Negative Bacteria
Base Pairing
Genes
Amino Acid Sequence
Proteins
colanic acid

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

RcsB and RcsC : A two-component regulator of capsule synthesis in Escherichia coli. / Stout, V.; Gottesman, S.

In: Journal of Bacteriology, Vol. 172, No. 2, 1990, p. 659-669.

Research output: Contribution to journalArticle

Stout, V. ; Gottesman, S. / RcsB and RcsC : A two-component regulator of capsule synthesis in Escherichia coli. In: Journal of Bacteriology. 1990 ; Vol. 172, No. 2. pp. 659-669.
@article{b0e7facac1ff4369b8c390b70127b605,
title = "RcsB and RcsC: A two-component regulator of capsule synthesis in Escherichia coli",
abstract = "Colanic acid capsule synthesis in Escherichia coli K-12 is regulated by RcsB and RcsC. The amino acid sequences of these two proteins, deduced from the nucleotide sequence reported here, demonstrate their homology to environmentally responsive two-component regulators that have been reported in both gram-positive and gram-negative bacteria. In our model, RcsC acts as the sensor and RcsB acts as the receiver or effector to stimulate capsule synthesis from cps genes. In addition, RcsC shows limited homology to the other effectors in its C terminus. Fusions of rscC to phoA that resulted in PhoA+ strains demonstrated that RcsC is a transmembrane protein with a periplasmic N-terminal domain and cytoplasmic C-terminal domain. Additional control of this regulatory network is provided by the dependence on the alternate sigma factor, RpoN, for the synthesis of RcsB. The rcsB and rcsC genes, which are oriented convergently with their stop codons 196 base pairs apart, are separated by a long direct repeat including two repetitive extragenic palindromic sequences.",
author = "V. Stout and S. Gottesman",
year = "1990",
language = "English (US)",
volume = "172",
pages = "659--669",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "2",

}

TY - JOUR

T1 - RcsB and RcsC

T2 - A two-component regulator of capsule synthesis in Escherichia coli

AU - Stout, V.

AU - Gottesman, S.

PY - 1990

Y1 - 1990

N2 - Colanic acid capsule synthesis in Escherichia coli K-12 is regulated by RcsB and RcsC. The amino acid sequences of these two proteins, deduced from the nucleotide sequence reported here, demonstrate their homology to environmentally responsive two-component regulators that have been reported in both gram-positive and gram-negative bacteria. In our model, RcsC acts as the sensor and RcsB acts as the receiver or effector to stimulate capsule synthesis from cps genes. In addition, RcsC shows limited homology to the other effectors in its C terminus. Fusions of rscC to phoA that resulted in PhoA+ strains demonstrated that RcsC is a transmembrane protein with a periplasmic N-terminal domain and cytoplasmic C-terminal domain. Additional control of this regulatory network is provided by the dependence on the alternate sigma factor, RpoN, for the synthesis of RcsB. The rcsB and rcsC genes, which are oriented convergently with their stop codons 196 base pairs apart, are separated by a long direct repeat including two repetitive extragenic palindromic sequences.

AB - Colanic acid capsule synthesis in Escherichia coli K-12 is regulated by RcsB and RcsC. The amino acid sequences of these two proteins, deduced from the nucleotide sequence reported here, demonstrate their homology to environmentally responsive two-component regulators that have been reported in both gram-positive and gram-negative bacteria. In our model, RcsC acts as the sensor and RcsB acts as the receiver or effector to stimulate capsule synthesis from cps genes. In addition, RcsC shows limited homology to the other effectors in its C terminus. Fusions of rscC to phoA that resulted in PhoA+ strains demonstrated that RcsC is a transmembrane protein with a periplasmic N-terminal domain and cytoplasmic C-terminal domain. Additional control of this regulatory network is provided by the dependence on the alternate sigma factor, RpoN, for the synthesis of RcsB. The rcsB and rcsC genes, which are oriented convergently with their stop codons 196 base pairs apart, are separated by a long direct repeat including two repetitive extragenic palindromic sequences.

UR - http://www.scopus.com/inward/record.url?scp=0025095229&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025095229&partnerID=8YFLogxK

M3 - Article

C2 - 2404948

AN - SCOPUS:0025095229

VL - 172

SP - 659

EP - 669

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 2

ER -