Rapid Tryptic Mapping Using Enzymically Active Mass Spectrometer Probe Tips

David Dogruel, Peter Williams, Randall W. Nelson

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

A method has been developed for rapid, sensitive, and accurate tryptic mapping of polypeptides using matrixassisted laser desorption/ionization time-of-flight mass analysis. The technique utilizes mass spectrometer probe tips which have been activated through the covalent immobilization of txypsin. The enzymatically active probe tips were used for the tryptic mapping of chicken egg lysozyme and the results compared with those obtained using either free trypsin or agarose-immobilized trypsin. A significant increase in the overall sensitivity of the process was observed using the active probe tips, as well as the production of more characteristic proteolytic fragments and the elimination of background signals due to the autolysis of the trypsin. Further, probe tip digestions were found to be rapid and convenient.

Original languageEnglish (US)
Pages (from-to)4343-4348
Number of pages6
JournalAnalytical chemistry
Volume67
Issue number23
DOIs
StatePublished - Jan 1 1995

ASJC Scopus subject areas

  • Analytical Chemistry

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