Rapid selection of accessible and cleavable sites in RNA by Escherichia coli RNase P and random external guide sequences

Eirik W. Lundblad, Gaoping Xiao, Jae Hyeong Ko, Sidney Altman

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

A method of inhibiting the expression of particular genes by using external guide sequences (EGSs) has been improved in its rapidity and specificity. Random EGSs that have 14-nt random sequences are used in the selection procedure for an EGS that attacks the mRNA for a gene in a particular location. A mixture of the random EGSs, the particular target RNA, and RNase P is used in the diagnostic procedure, which, after completion, is analyzed in a gel with suitable control lanes. Within a few hours, the procedure is complete. The action of EGSs designed by an older method is compared with EGSs designed by the random EGS method on mRNAs from two bacterial pathogens.

Original languageEnglish (US)
Pages (from-to)2354-2357
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number7
DOIs
StatePublished - Feb 19 2008
Externally publishedYes

ASJC Scopus subject areas

  • General

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