TY - JOUR
T1 - Rapid selection of accessible and cleavable sites in RNA by Escherichia coli RNase P and random external guide sequences
AU - Lundblad, Eirik W.
AU - Xiao, Gaoping
AU - Ko, Jae Hyeong
AU - Altman, Sidney
PY - 2008/2/19
Y1 - 2008/2/19
N2 - A method of inhibiting the expression of particular genes by using external guide sequences (EGSs) has been improved in its rapidity and specificity. Random EGSs that have 14-nt random sequences are used in the selection procedure for an EGS that attacks the mRNA for a gene in a particular location. A mixture of the random EGSs, the particular target RNA, and RNase P is used in the diagnostic procedure, which, after completion, is analyzed in a gel with suitable control lanes. Within a few hours, the procedure is complete. The action of EGSs designed by an older method is compared with EGSs designed by the random EGS method on mRNAs from two bacterial pathogens.
AB - A method of inhibiting the expression of particular genes by using external guide sequences (EGSs) has been improved in its rapidity and specificity. Random EGSs that have 14-nt random sequences are used in the selection procedure for an EGS that attacks the mRNA for a gene in a particular location. A mixture of the random EGSs, the particular target RNA, and RNase P is used in the diagnostic procedure, which, after completion, is analyzed in a gel with suitable control lanes. Within a few hours, the procedure is complete. The action of EGSs designed by an older method is compared with EGSs designed by the random EGS method on mRNAs from two bacterial pathogens.
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U2 - 10.1073/pnas.0711977105
DO - 10.1073/pnas.0711977105
M3 - Article
C2 - 18263737
AN - SCOPUS:40649105539
SN - 0027-8424
VL - 105
SP - 2354
EP - 2357
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 7
ER -