Rapid protein characterization using bioreactive mass spectrometer probe tips

Randall W. Nelson; J. Kathleen M Lewis; David Dogruel; Jennifer R. Krone; Peter Williams

Rapid protein characterization using bioreactive mass spectrometer probe tips

Randall W. Nelson, J. Kathleen M Lewis, David Dogruel, Jennifer R. Krone, Peter Williams

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Methods have been developed for the rapid and sensitive mass spectrometric characterization of peptides. The approach uses bioreactive mass spectrometer probe tips which are capable of modifying proteins for analytical purpose. In the demonstrated case, enzymatic proteolysis is initiated upon application of analyte to the probe tips, time allowed for digestion, and the results analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The probe tips have been used for proteolytic mapping and partial sequence determination of picomol quantities of peptide while maintaining analysis times of approximately 5 minutes.

Original language	English (US)
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering
Editors	Gerald E. Cohn, Steven A. Soper, C.H.W. Chen
Pages	390-396
Number of pages	7
State	Published - 1996
Event	Ultrasensitive Biochemical Diagnostics - San Jose, CA, USA Duration: Jan 31 1996 → Feb 2 1996

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	2680
ISSN (Print)	0277-786X

Other

Other	Ultrasensitive Biochemical Diagnostics
City	San Jose, CA, USA
Period	1/31/96 → 2/2/96

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Cite this

Nelson, R. W., Lewis, J. K. M., Dogruel, D., Krone, J. R., & Williams, P. (1996). Rapid protein characterization using bioreactive mass spectrometer probe tips. In G. E. Cohn, S. A. Soper, & C. H. W. Chen (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (pp. 390-396). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 2680).

Rapid protein characterization using bioreactive mass spectrometer probe tips. / Nelson, Randall W.; Lewis, J. Kathleen M; Dogruel, David et al.
Proceedings of SPIE - The International Society for Optical Engineering. ed. / Gerald E. Cohn; Steven A. Soper; C.H.W. Chen. 1996. p. 390-396 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 2680).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Nelson, RW, Lewis, JKM, Dogruel, D, Krone, JR & Williams, P 1996, Rapid protein characterization using bioreactive mass spectrometer probe tips. in GE Cohn, SA Soper & CHW Chen (eds), Proceedings of SPIE - The International Society for Optical Engineering. Proceedings of SPIE - The International Society for Optical Engineering, vol. 2680, pp. 390-396, Ultrasensitive Biochemical Diagnostics, San Jose, CA, USA, 1/31/96.

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title = "Rapid protein characterization using bioreactive mass spectrometer probe tips",

abstract = "Methods have been developed for the rapid and sensitive mass spectrometric characterization of peptides. The approach uses bioreactive mass spectrometer probe tips which are capable of modifying proteins for analytical purpose. In the demonstrated case, enzymatic proteolysis is initiated upon application of analyte to the probe tips, time allowed for digestion, and the results analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The probe tips have been used for proteolytic mapping and partial sequence determination of picomol quantities of peptide while maintaining analysis times of approximately 5 minutes.",

author = "Nelson, {Randall W.} and Lewis, {J. Kathleen M} and David Dogruel and Krone, {Jennifer R.} and Peter Williams",

year = "1996",

language = "English (US)",

isbn = "0819420549",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

pages = "390--396",

editor = "Cohn, {Gerald E.} and Soper, {Steven A.} and C.H.W. Chen",

booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",

note = "Ultrasensitive Biochemical Diagnostics ; Conference date: 31-01-1996 Through 02-02-1996",

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T1 - Rapid protein characterization using bioreactive mass spectrometer probe tips

AU - Nelson, Randall W.

AU - Lewis, J. Kathleen M

AU - Dogruel, David

AU - Krone, Jennifer R.

AU - Williams, Peter

PY - 1996

Y1 - 1996

N2 - Methods have been developed for the rapid and sensitive mass spectrometric characterization of peptides. The approach uses bioreactive mass spectrometer probe tips which are capable of modifying proteins for analytical purpose. In the demonstrated case, enzymatic proteolysis is initiated upon application of analyte to the probe tips, time allowed for digestion, and the results analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The probe tips have been used for proteolytic mapping and partial sequence determination of picomol quantities of peptide while maintaining analysis times of approximately 5 minutes.

AB - Methods have been developed for the rapid and sensitive mass spectrometric characterization of peptides. The approach uses bioreactive mass spectrometer probe tips which are capable of modifying proteins for analytical purpose. In the demonstrated case, enzymatic proteolysis is initiated upon application of analyte to the probe tips, time allowed for digestion, and the results analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The probe tips have been used for proteolytic mapping and partial sequence determination of picomol quantities of peptide while maintaining analysis times of approximately 5 minutes.

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M3 - Conference contribution

AN - SCOPUS:0029722277

SN - 0819420549

SN - 9780819420541

T3 - Proceedings of SPIE - The International Society for Optical Engineering

SP - 390

EP - 396

BT - Proceedings of SPIE - The International Society for Optical Engineering

A2 - Cohn, Gerald E.

A2 - Soper, Steven A.

A2 - Chen, C.H.W.

T2 - Ultrasensitive Biochemical Diagnostics

Y2 - 31 January 1996 through 2 February 1996

ER -

Rapid protein characterization using bioreactive mass spectrometer probe tips

Abstract

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ASJC Scopus subject areas

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