Rapid protein characterization using bioreactive mass spectrometer probe tips

Randall W. Nelson, J. Kathleen M Lewis, David Dogruel, Jennifer R. Krone, Peter Williams

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Methods have been developed for the rapid and sensitive mass spectrometric characterization of peptides. The approach uses bioreactive mass spectrometer probe tips which are capable of modifying proteins for analytical purpose. In the demonstrated case, enzymatic proteolysis is initiated upon application of analyte to the probe tips, time allowed for digestion, and the results analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The probe tips have been used for proteolytic mapping and partial sequence determination of picomol quantities of peptide while maintaining analysis times of approximately 5 minutes.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsGerald E. Cohn, Steven A. Soper, C.H.W. Chen
Pages390-396
Number of pages7
Volume2680
StatePublished - 1996
EventUltrasensitive Biochemical Diagnostics - San Jose, CA, USA
Duration: Jan 31 1996Feb 2 1996

Other

OtherUltrasensitive Biochemical Diagnostics
CitySan Jose, CA, USA
Period1/31/962/2/96

Fingerprint

Mass spectrometers
mass spectrometers
proteins
Proteins
Peptides
peptides
probes
Proteolysis
Ionization
Mass spectrometry
Desorption
mass spectroscopy
desorption
ionization
Lasers
matrices
lasers

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Nelson, R. W., Lewis, J. K. M., Dogruel, D., Krone, J. R., & Williams, P. (1996). Rapid protein characterization using bioreactive mass spectrometer probe tips. In G. E. Cohn, S. A. Soper, & C. H. W. Chen (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 2680, pp. 390-396)

Rapid protein characterization using bioreactive mass spectrometer probe tips. / Nelson, Randall W.; Lewis, J. Kathleen M; Dogruel, David; Krone, Jennifer R.; Williams, Peter.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / Gerald E. Cohn; Steven A. Soper; C.H.W. Chen. Vol. 2680 1996. p. 390-396.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nelson, RW, Lewis, JKM, Dogruel, D, Krone, JR & Williams, P 1996, Rapid protein characterization using bioreactive mass spectrometer probe tips. in GE Cohn, SA Soper & CHW Chen (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 2680, pp. 390-396, Ultrasensitive Biochemical Diagnostics, San Jose, CA, USA, 1/31/96.
Nelson RW, Lewis JKM, Dogruel D, Krone JR, Williams P. Rapid protein characterization using bioreactive mass spectrometer probe tips. In Cohn GE, Soper SA, Chen CHW, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2680. 1996. p. 390-396
Nelson, Randall W. ; Lewis, J. Kathleen M ; Dogruel, David ; Krone, Jennifer R. ; Williams, Peter. / Rapid protein characterization using bioreactive mass spectrometer probe tips. Proceedings of SPIE - The International Society for Optical Engineering. editor / Gerald E. Cohn ; Steven A. Soper ; C.H.W. Chen. Vol. 2680 1996. pp. 390-396
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