Rapid protein characterization using bioreactive mass spectrometer probe tips

Randall W. Nelson, J. Kathleen M Lewis, David Dogruel, Jennifer R. Krone, Peter Williams

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

Methods have been developed for the rapid and sensitive mass spectrometric characterization of peptides. The approach uses bioreactive mass spectrometer probe tips which are capable of modifying proteins for analytical purpose. In the demonstrated case, enzymatic proteolysis is initiated upon application of analyte to the probe tips, time allowed for digestion, and the results analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The probe tips have been used for proteolytic mapping and partial sequence determination of picomol quantities of peptide while maintaining analysis times of approximately 5 minutes.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsGerald E. Cohn, Steven A. Soper, C.H.W. Chen
Pages390-396
Number of pages7
StatePublished - Jan 1 1996
EventUltrasensitive Biochemical Diagnostics - San Jose, CA, USA
Duration: Jan 31 1996Feb 2 1996

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume2680
ISSN (Print)0277-786X

Other

OtherUltrasensitive Biochemical Diagnostics
CitySan Jose, CA, USA
Period1/31/962/2/96

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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