Rapid nonlinear image scanning microscopy

Ingo Gregor; Martin Spiecker; Roman Petrovsky; Jörg Großhans; Robert Ros; Jörg Enderlein

doi:10.1038/nmeth.4467

Rapid nonlinear image scanning microscopy

Ingo Gregor, Martin Spiecker, Roman Petrovsky, Jörg Großhans, Robert Ros, Jörg Enderlein

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

Image scanning microscopy (ISM) doubles the resolution of a conventional confocal microscope for super-resolution imaging. Here, we describe an all-optical ISM design based on rescanning microscopy for two-photon-excited fluorescence and second-harmonic generation that allows straightforward implementation into existing microscopes. The design offers improved sensitivity and high frame rates relative to those of existing systems. We demonstrate its utility using fixed and living specimens as well as collagen hydrogels.

Original language	English (US)
Pages (from-to)	1087-1089
Number of pages	3
Journal	Nature Methods
Volume	14
Issue number	11
DOIs	https://doi.org/10.1038/nmeth.4467
State	Published - Oct 31 2017

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth.4467

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title = "Rapid nonlinear image scanning microscopy",

abstract = "Image scanning microscopy (ISM) doubles the resolution of a conventional confocal microscope for super-resolution imaging. Here, we describe an all-optical ISM design based on rescanning microscopy for two-photon-excited fluorescence and second-harmonic generation that allows straightforward implementation into existing microscopes. The design offers improved sensitivity and high frame rates relative to those of existing systems. We demonstrate its utility using fixed and living specimens as well as collagen hydrogels.",

author = "Ingo Gregor and Martin Spiecker and Roman Petrovsky and J{\"o}rg Gro{\ss}hans and Robert Ros and J{\"o}rg Enderlein",

note = "Funding Information: Part of this work (J.E., I.G.) was supported by the Cluster of Excellence and DFG Research Center Nanoscale Microscopy and Molecular Physiology of the Brain. R.R. was supported by the NSF grant 1510700. We thank F. Rehfeldt (3rd Institute of Physics-Biophysics, University of G{\"o}ttingen, G{\"o}ttingen, Germany) for the hMSC samples, and J. Faust for help with the Arivis image processing.",

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AU - Gregor, Ingo

AU - Spiecker, Martin

AU - Petrovsky, Roman

AU - Großhans, Jörg

AU - Ros, Robert

AU - Enderlein, Jörg

N1 - Funding Information: Part of this work (J.E., I.G.) was supported by the Cluster of Excellence and DFG Research Center Nanoscale Microscopy and Molecular Physiology of the Brain. R.R. was supported by the NSF grant 1510700. We thank F. Rehfeldt (3rd Institute of Physics-Biophysics, University of Göttingen, Göttingen, Germany) for the hMSC samples, and J. Faust for help with the Arivis image processing.

PY - 2017/10/31

Y1 - 2017/10/31

N2 - Image scanning microscopy (ISM) doubles the resolution of a conventional confocal microscope for super-resolution imaging. Here, we describe an all-optical ISM design based on rescanning microscopy for two-photon-excited fluorescence and second-harmonic generation that allows straightforward implementation into existing microscopes. The design offers improved sensitivity and high frame rates relative to those of existing systems. We demonstrate its utility using fixed and living specimens as well as collagen hydrogels.

AB - Image scanning microscopy (ISM) doubles the resolution of a conventional confocal microscope for super-resolution imaging. Here, we describe an all-optical ISM design based on rescanning microscopy for two-photon-excited fluorescence and second-harmonic generation that allows straightforward implementation into existing microscopes. The design offers improved sensitivity and high frame rates relative to those of existing systems. We demonstrate its utility using fixed and living specimens as well as collagen hydrogels.

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Rapid nonlinear image scanning microscopy

Abstract

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