Abstract
Endothelial cells (ECs) form the inner lining of the blood vasculature and are therefore exposed to shear stress (tau), the tangential component of hemodynamic forces. Shear stress exacts potent changes in EC biology, possibly via EC-membrane perturbations, with consequent influences on vascular health. Here we describe a time-correlated single photon counting method for measuring rapid and spatially heterogeneous changes in fluorescence lifetimes and polarizations of EC membranes exposed to shear stress. The role in mechanotransduction by ECs of shear-induced changes in membrane lipid dynamics is discussed.
| Original language | English (US) |
|---|---|
| Title of host publication | Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings |
| Pages | 320 |
| Number of pages | 1 |
| Volume | 1 |
| State | Published - 2002 |
| Event | Proceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States Duration: Oct 23 2002 → Oct 26 2002 |
Other
| Other | Proceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) |
|---|---|
| Country/Territory | United States |
| City | Houston, TX |
| Period | 10/23/02 → 10/26/02 |
Keywords
- Endothelium
- Fluorescence lifetime
- Polarization
- Shear
- Spectroscopy
ASJC Scopus subject areas
- Bioengineering