Rapid, multipoint molecular spectroscopic measurements of shear effects on endothelial cell membranes

Peter J. Butler, Benjamin P. Bowen, Neal Woodbury

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Endothelial cells (ECs) form the inner lining of the blood vasculature and are therefore exposed to shear stress (tau), the tangential component of hemodynamic forces. Shear stress exacts potent changes in EC biology, possibly via EC-membrane perturbations, with consequent influences on vascular health. Here we describe a time-correlated single photon counting method for measuring rapid and spatially heterogeneous changes in fluorescence lifetimes and polarizations of EC membranes exposed to shear stress. The role in mechanotransduction by ECs of shear-induced changes in membrane lipid dynamics is discussed.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Pages320
Number of pages1
Volume1
StatePublished - 2002
EventProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States
Duration: Oct 23 2002Oct 26 2002

Other

OtherProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS)
CountryUnited States
CityHouston, TX
Period10/23/0210/26/02

Keywords

  • Endothelium
  • Fluorescence lifetime
  • Polarization
  • Shear
  • Spectroscopy

ASJC Scopus subject areas

  • Bioengineering

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  • Cite this

    Butler, P. J., Bowen, B. P., & Woodbury, N. (2002). Rapid, multipoint molecular spectroscopic measurements of shear effects on endothelial cell membranes. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 1, pp. 320)