Rapid detection of a dengue virus RNA sequence with single molecule sensitivity using tandem toehold-mediated displacement reactions

Mingxuan Gao; Douglas Daniel; Hongyan Zou; Shuoxing Jiang; Su Lin; Chengzhi Huang; Sidney Hecht; Shengxi Chen

doi:10.1039/c7cc09131a

Rapid detection of a dengue virus RNA sequence with single molecule sensitivity using tandem toehold-mediated displacement reactions

Mingxuan Gao, Douglas Daniel, Hongyan Zou, Shuoxing Jiang, Su Lin, Chengzhi Huang, Sidney Hecht, Shengxi Chen

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

A DNA tetrahedron having a single-strand DNA edge was used to detect a dengue virus RNA sequence. Novel tandem toehold-mediated displacement reactions (tTMDR) were developed to amplify the fluorescence signal from the DNA tetrahedron. Using an excess of the DNA tetrahedron each target RNA was recycled about 10³ times during the tTMDR process. This amplification process was used for the sensitive detection of dengue virus RNA in this study. As few as 6 copies of RNA per sample could be detected using a photon count technique with single molecule sensitivity.

Original language	English (US)
Pages (from-to)	968-971
Number of pages	4
Journal	Chemical Communications
Volume	54
Issue number	8
DOIs	https://doi.org/10.1039/c7cc09131a
State	Published - 2018

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/c7cc09131a

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title = "Rapid detection of a dengue virus RNA sequence with single molecule sensitivity using tandem toehold-mediated displacement reactions",

abstract = "A DNA tetrahedron having a single-strand DNA edge was used to detect a dengue virus RNA sequence. Novel tandem toehold-mediated displacement reactions (tTMDR) were developed to amplify the fluorescence signal from the DNA tetrahedron. Using an excess of the DNA tetrahedron each target RNA was recycled about 103 times during the tTMDR process. This amplification process was used for the sensitive detection of dengue virus RNA in this study. As few as 6 copies of RNA per sample could be detected using a photon count technique with single molecule sensitivity.",

author = "Mingxuan Gao and Douglas Daniel and Hongyan Zou and Shuoxing Jiang and Su Lin and Chengzhi Huang and Sidney Hecht and Shengxi Chen",

note = "Funding Information: The US Army Peer Reviewed Medical Research Program (W81XWH-16-1-0141) supported this work. Publisher Copyright: {\textcopyright} 2018 The Royal Society of Chemistry.",

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T1 - Rapid detection of a dengue virus RNA sequence with single molecule sensitivity using tandem toehold-mediated displacement reactions

AU - Gao, Mingxuan

AU - Daniel, Douglas

AU - Zou, Hongyan

AU - Jiang, Shuoxing

AU - Lin, Su

AU - Huang, Chengzhi

AU - Hecht, Sidney

AU - Chen, Shengxi

PY - 2018

Y1 - 2018

N2 - A DNA tetrahedron having a single-strand DNA edge was used to detect a dengue virus RNA sequence. Novel tandem toehold-mediated displacement reactions (tTMDR) were developed to amplify the fluorescence signal from the DNA tetrahedron. Using an excess of the DNA tetrahedron each target RNA was recycled about 103 times during the tTMDR process. This amplification process was used for the sensitive detection of dengue virus RNA in this study. As few as 6 copies of RNA per sample could be detected using a photon count technique with single molecule sensitivity.

AB - A DNA tetrahedron having a single-strand DNA edge was used to detect a dengue virus RNA sequence. Novel tandem toehold-mediated displacement reactions (tTMDR) were developed to amplify the fluorescence signal from the DNA tetrahedron. Using an excess of the DNA tetrahedron each target RNA was recycled about 103 times during the tTMDR process. This amplification process was used for the sensitive detection of dengue virus RNA in this study. As few as 6 copies of RNA per sample could be detected using a photon count technique with single molecule sensitivity.

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JO - Chemical Communications

JF - Chemical Communications

IS - 8

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Rapid detection of a dengue virus RNA sequence with single molecule sensitivity using tandem toehold-mediated displacement reactions

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