Rapid detection of a dengue virus RNA sequence with single molecule sensitivity using tandem toehold-mediated displacement reactions

Mingxuan Gao, Douglas Daniel, Hongyan Zou, Shuoxing Jiang, Su Lin, Chengzhi Huang, Sidney Hecht, Shengxi Chen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A DNA tetrahedron having a single-strand DNA edge was used to detect a dengue virus RNA sequence. Novel tandem toehold-mediated displacement reactions (tTMDR) were developed to amplify the fluorescence signal from the DNA tetrahedron. Using an excess of the DNA tetrahedron each target RNA was recycled about 103 times during the tTMDR process. This amplification process was used for the sensitive detection of dengue virus RNA in this study. As few as 6 copies of RNA per sample could be detected using a photon count technique with single molecule sensitivity.

Original languageEnglish (US)
Pages (from-to)968-971
Number of pages4
JournalChemical Communications
Volume54
Issue number8
DOIs
StatePublished - Jan 1 2018

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RNA
Viruses
DNA
Molecules
Amplification
Photons
Fluorescence

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

Rapid detection of a dengue virus RNA sequence with single molecule sensitivity using tandem toehold-mediated displacement reactions. / Gao, Mingxuan; Daniel, Douglas; Zou, Hongyan; Jiang, Shuoxing; Lin, Su; Huang, Chengzhi; Hecht, Sidney; Chen, Shengxi.

In: Chemical Communications, Vol. 54, No. 8, 01.01.2018, p. 968-971.

Research output: Contribution to journalArticle

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