Rapid decay of tumoricidal activity and loss of responsiveness to lymphokines in inflammatory macrophages

George Poste, R. Kirsh

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The ability of inflammatory tissue macrophages harvested on glass coverslips implanted in the s.c. tissue of C57BL/6 mice to kill tumor cells in vitro has been examined. Macrophages present on coverslips implanted for less than 4 days are devoid of spontaneous tumoricidal activity but can be rendered cytotoxic for syngeneic and allogeneic tumor cells in vitro by incubation in vitro with lymphokines released by mitogen-stimulated lymphocytes. Inflammatory macrophages on coverslips implanted for 4 to 7 days show significant spontaneous cytotoxicity for tumor cells in vitro, and their tumoricidal activity is further increased by additional incubation in vitro with lymphokines. With progression, the inflammatory macrophages harvested on coverslips implanted for longer than 7 days lack spontaneous cytotoxic activity and are also resistant to activation by lymphokines in vitro. These alterations in tumoricidal activity and responsiveness to lymphokines are accompanied by a marked reduction in the number of peroxidase-positive macrophages within the population, suggesting that maintenance of tumoricidal activity requires continuous influx of new peroxidase-positive macrophages from the circulation. Previously activated macrophages which have lost their tumoricidal activity and become refractory to reactivation by lymphokines in the extracellular environment can be reactivated by treatment in vitro with liposomes containing encapsulated lymphokines.

Original languageEnglish (US)
Pages (from-to)2582-2590
Number of pages9
JournalCancer Research
Volume39
Issue number7 I
StatePublished - 1979
Externally publishedYes

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Lymphokines
Macrophages
Peroxidase
Neoplasms
In Vitro Techniques
Inbred C57BL Mouse
Mitogens
Liposomes
Glass
Maintenance
Lymphocytes
Population

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Rapid decay of tumoricidal activity and loss of responsiveness to lymphokines in inflammatory macrophages. / Poste, George; Kirsh, R.

In: Cancer Research, Vol. 39, No. 7 I, 1979, p. 2582-2590.

Research output: Contribution to journalArticle

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