Rapid chlorophyll a fluorescence transient of Lemna gibba leaf as an indication of light and hydroxylamine effect on photosystem II activity

David Dewez, Nadia Ait Ali, Francois Perreault, Radovan Popovic

Research output: Contribution to journalArticle

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Abstract

Rapid chlorophyll fluorescence transient induced by saturating flash (3000 μmol of photons m-2 s-1) was investigated when Lemna gibba had been exposed to light (100 μmol of photons m-2 s -1) causing the Kautsky effect or in low light intensity unable to trigger PSII photochemistry. Measurements were made by using, simultaneously, a pulse amplitude modulated fluorometer and plant efficiency analyzer system, either on non-treated L. gibba leaf or those treated with different concentrations of hydroxylamine (1-50 mM) causing gradual inhibition of the water splitting system. When any leaf was exposed to continuous light during the Kautsky effect, a rapid fluorescence transient may reflect current activity of photosystem II within the photosystem II complex. Under those conditions, a variation of transition steps appearing over time was related to a drastic change to the photosystem II functional properties. This value indicated that the energy dissipation through non-photochemical pathways was undergoing extreme change. The change of rapid fluorescence transient, induced under continuous light, when compared to those obtained under very low light intensity, confirmed the ability of photosystem II to be capable to undergo rapid adaptation lasting about two minutes. When the water splitting system was inhibited and electron donation partially substituted by hydroxylamine, the adaptation ability of photosystem II to different light conditions was lost. In this study, the change of rapid fluorescence kinetic and transient appearing over time was shown to be a good indication for the change of the functional properties of photosystem II induced either by light or by hydroxylamine.

Original languageEnglish (US)
Pages (from-to)532-538
Number of pages7
JournalPhotochemical and Photobiological Sciences
Volume6
Issue number5
DOIs
StatePublished - 2007
Externally publishedYes

Fingerprint

Hydroxylamine
Photosystem II Protein Complex
chlorophylls
leaves
indication
Fluorescence
Light
fluorescence
water splitting
luminous intensity
Photons
photons
pulse amplitude
Fluorometers
photochemical reactions
Photochemistry
flash
analyzers
Water
Photochemical reactions

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Cell Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Rapid chlorophyll a fluorescence transient of Lemna gibba leaf as an indication of light and hydroxylamine effect on photosystem II activity. / Dewez, David; Ali, Nadia Ait; Perreault, Francois; Popovic, Radovan.

In: Photochemical and Photobiological Sciences, Vol. 6, No. 5, 2007, p. 532-538.

Research output: Contribution to journalArticle

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