Rapid apolipoprotein phenotyping using mass spectrometric immunoassay approaches

Eric E. Niederkofler, Dobrin Nedelkov, Kemmons A. Tubbs, Urban A. Kiernan, Peter Williams, Randall W. Nelson

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Mass spectrometric immunoassay approaches were used for rapid apolipoprotein phenotyping. Affinity pipettes were prepared by immobilizing a specific anti-apolipoprotein antibody onto a small-volume porous frit fitted at the entrance of a wide bore P200 pipette tip. The samples were incubated against the affinity pipettes by repetitively drawing the samples through the pipettes. The proteins captured in the affinity pipettes were eluted onto MALDI-TOF mass spectrometry (MS) targets using acidified MALDI matrix solution.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages253-254
Number of pages2
StatePublished - 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

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Apolipoproteins
Mass spectrometry
Antibodies
Proteins

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Niederkofler, E. E., Nedelkov, D., Tubbs, K. A., Kiernan, U. A., Williams, P., & Nelson, R. W. (2002). Rapid apolipoprotein phenotyping using mass spectrometric immunoassay approaches. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 253-254)

Rapid apolipoprotein phenotyping using mass spectrometric immunoassay approaches. / Niederkofler, Eric E.; Nedelkov, Dobrin; Tubbs, Kemmons A.; Kiernan, Urban A.; Williams, Peter; Nelson, Randall W.

Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 253-254.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Niederkofler, EE, Nedelkov, D, Tubbs, KA, Kiernan, UA, Williams, P & Nelson, RW 2002, Rapid apolipoprotein phenotyping using mass spectrometric immunoassay approaches. in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 253-254, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.
Niederkofler EE, Nedelkov D, Tubbs KA, Kiernan UA, Williams P, Nelson RW. Rapid apolipoprotein phenotyping using mass spectrometric immunoassay approaches. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 253-254
Niederkofler, Eric E. ; Nedelkov, Dobrin ; Tubbs, Kemmons A. ; Kiernan, Urban A. ; Williams, Peter ; Nelson, Randall W. / Rapid apolipoprotein phenotyping using mass spectrometric immunoassay approaches. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. pp. 253-254
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