Rampant purifying selection conserves positions with posttranslational modifications in human proteins

Vanessa E. Gray, Sudhir Kumar

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Posttranslational modifications (PTMs) are chemical alterations that are critical to protein conformation and activation states. Despite their functional importance and reported involvement in many diseases, evolutionary analyses have produced enigmatic results because only weak or no selective pressures have been attributed to many types of PTMs. In a large-scale analysis of 16,836 PTM positions from 4,484 human proteins, we find that positions harboring PTMs show evidence of higher purifying selection in 70% of the phosphorylated and N-linked glycosylated proteins. The purifying selection is up to 42% more severe at PTM residues as compared with the corresponding unmodified amino acids. These results establish extensive selective pressures in the long-term history of positions that experience PTMs in the human proteins. Our findings will enhance our understanding of the historical function of PTMs over time and help in predicting PTM positions by using evolutionary comparisons.

Original languageEnglish (US)
Pages (from-to)1565-1568
Number of pages4
JournalMolecular biology and evolution
Volume28
Issue number5
DOIs
StatePublished - May 1 2011

Keywords

  • evolution
  • genomics
  • posttranslational modifications
  • proteomics

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

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