RAF2 is a RuBisCO assembly factor in Arabidopsis thaliana

Rikard Fristedt, Chen Hu, Nicole Wheatley, Laura M. Roy, Rebekka Wachter, Linda Savage, Jeremy Harbinson, David M. Kramer, Sabeeha S. Merchant, Todd Yeates, Roberta Croce

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) catalyzes the reaction between gaseous carbon dioxide (CO 2 ) and ribulose-1,5-bisphosphate. Although it is one of the most studied enzymes, the assembly mechanisms of the large hexadecameric RuBisCO is still emerging. In bacteria and in the C4 plant Zea mays, a protein with distant homology to pterin-4α-carbinolamine dehydratase (PCD) has recently been shown to be involved in RuBisCO assembly. However, studies of the homologous PCD-like protein (RAF2, RuBisCO assembly factor 2) in the C3 plant Arabidopsis thaliana (A. thaliana) have so far focused on its role in hormone and stress signaling. We investigated whether A. thalianaRAF2 is also involved in RuBisCO assembly. We localized RAF2 to the soluble chloroplast stroma and demonstrated that raf2 A. thaliana mutant plants display a severe pale green phenotype with reduced levels of stromal RuBisCO. We concluded that the RAF2 protein is probably involved in RuBisCO assembly in the C3 plant A. thaliana.

Original languageEnglish (US)
Pages (from-to)146-156
Number of pages11
JournalPlant Journal
Volume94
Issue number1
DOIs
StatePublished - Apr 2018

Keywords

  • Atg5 g51110
  • RAF2
  • RuBisCO
  • RuBisCO aggregation
  • RuBisCO assembly factor
  • SDIRIP1
  • abscisic acid
  • chloroplast

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

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  • Cite this

    Fristedt, R., Hu, C., Wheatley, N., Roy, L. M., Wachter, R., Savage, L., Harbinson, J., Kramer, D. M., Merchant, S. S., Yeates, T., & Croce, R. (2018). RAF2 is a RuBisCO assembly factor in Arabidopsis thaliana. Plant Journal, 94(1), 146-156. https://doi.org/10.1111/tpj.13849