RAF2 is a RuBisCO assembly factor in Arabidopsis thaliana

Rikard Fristedt, Chen Hu, Nicole Wheatley, Laura M. Roy, Rebekka Wachter, Linda Savage, Jeremy Harbinson, David M. Kramer, Sabeeha S. Merchant, Todd Yeates, Roberta Croce

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) catalyzes the reaction between gaseous carbon dioxide (CO2) and ribulose-1,5-bisphosphate. Although it is one of the most studied enzymes, the assembly mechanisms of the large hexadecameric RuBisCO is still emerging. In bacteria and in the C4 plant Zea mays, a protein with distant homology to pterin-4α-carbinolamine dehydratase (PCD) has recently been shown to be involved in RuBisCO assembly. However, studies of the homologous PCD-like protein (RAF2, RuBisCO assembly factor 2) in the C3 plant Arabidopsis thaliana (A. thaliana) have so far focused on its role in hormone and stress signaling. We investigated whether A. thalianaRAF2 is also involved in RuBisCO assembly. We localized RAF2 to the soluble chloroplast stroma and demonstrated that raf2 A. thaliana mutant plants display a severe pale green phenotype with reduced levels of stromal RuBisCO. We concluded that the RAF2 protein is probably involved in RuBisCO assembly in the C3 plant A. thaliana.

Original languageEnglish (US)
Pages (from-to)146-156
Number of pages11
JournalPlant Journal
Volume94
Issue number1
DOIs
StatePublished - Apr 1 2018

Fingerprint

Oxygenases
ribulose 1,5-diphosphate
oxygenases
Arabidopsis
Arabidopsis thaliana
Pterins
Hydro-Lyases
C3 plants
carbon dioxide
Proteins
proteins
ribulose-1,5 diphosphate
C4 plants
Chloroplasts
Carbon Dioxide
Zea mays
chloroplasts
hormones
Hormones
Bacteria

Keywords

  • abscisic acid
  • Atg5 g51110
  • chloroplast
  • RAF2
  • RuBisCO
  • RuBisCO aggregation
  • RuBisCO assembly factor
  • SDIRIP1

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Fristedt, R., Hu, C., Wheatley, N., Roy, L. M., Wachter, R., Savage, L., ... Croce, R. (2018). RAF2 is a RuBisCO assembly factor in Arabidopsis thaliana. Plant Journal, 94(1), 146-156. https://doi.org/10.1111/tpj.13849

RAF2 is a RuBisCO assembly factor in Arabidopsis thaliana. / Fristedt, Rikard; Hu, Chen; Wheatley, Nicole; Roy, Laura M.; Wachter, Rebekka; Savage, Linda; Harbinson, Jeremy; Kramer, David M.; Merchant, Sabeeha S.; Yeates, Todd; Croce, Roberta.

In: Plant Journal, Vol. 94, No. 1, 01.04.2018, p. 146-156.

Research output: Contribution to journalArticle

Fristedt, R, Hu, C, Wheatley, N, Roy, LM, Wachter, R, Savage, L, Harbinson, J, Kramer, DM, Merchant, SS, Yeates, T & Croce, R 2018, 'RAF2 is a RuBisCO assembly factor in Arabidopsis thaliana' Plant Journal, vol. 94, no. 1, pp. 146-156. https://doi.org/10.1111/tpj.13849
Fristedt R, Hu C, Wheatley N, Roy LM, Wachter R, Savage L et al. RAF2 is a RuBisCO assembly factor in Arabidopsis thaliana. Plant Journal. 2018 Apr 1;94(1):146-156. https://doi.org/10.1111/tpj.13849
Fristedt, Rikard ; Hu, Chen ; Wheatley, Nicole ; Roy, Laura M. ; Wachter, Rebekka ; Savage, Linda ; Harbinson, Jeremy ; Kramer, David M. ; Merchant, Sabeeha S. ; Yeates, Todd ; Croce, Roberta. / RAF2 is a RuBisCO assembly factor in Arabidopsis thaliana. In: Plant Journal. 2018 ; Vol. 94, No. 1. pp. 146-156.
@article{1bf1edcc6ab54297bfbf25cdf59aed8b,
title = "RAF2 is a RuBisCO assembly factor in Arabidopsis thaliana",
abstract = "Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) catalyzes the reaction between gaseous carbon dioxide (CO2) and ribulose-1,5-bisphosphate. Although it is one of the most studied enzymes, the assembly mechanisms of the large hexadecameric RuBisCO is still emerging. In bacteria and in the C4 plant Zea mays, a protein with distant homology to pterin-4α-carbinolamine dehydratase (PCD) has recently been shown to be involved in RuBisCO assembly. However, studies of the homologous PCD-like protein (RAF2, RuBisCO assembly factor 2) in the C3 plant Arabidopsis thaliana (A. thaliana) have so far focused on its role in hormone and stress signaling. We investigated whether A. thalianaRAF2 is also involved in RuBisCO assembly. We localized RAF2 to the soluble chloroplast stroma and demonstrated that raf2 A. thaliana mutant plants display a severe pale green phenotype with reduced levels of stromal RuBisCO. We concluded that the RAF2 protein is probably involved in RuBisCO assembly in the C3 plant A. thaliana.",
keywords = "abscisic acid, Atg5 g51110, chloroplast, RAF2, RuBisCO, RuBisCO aggregation, RuBisCO assembly factor, SDIRIP1",
author = "Rikard Fristedt and Chen Hu and Nicole Wheatley and Roy, {Laura M.} and Rebekka Wachter and Linda Savage and Jeremy Harbinson and Kramer, {David M.} and Merchant, {Sabeeha S.} and Todd Yeates and Roberta Croce",
year = "2018",
month = "4",
day = "1",
doi = "10.1111/tpj.13849",
language = "English (US)",
volume = "94",
pages = "146--156",
journal = "Plant Journal",
issn = "0960-7412",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - RAF2 is a RuBisCO assembly factor in Arabidopsis thaliana

AU - Fristedt, Rikard

AU - Hu, Chen

AU - Wheatley, Nicole

AU - Roy, Laura M.

AU - Wachter, Rebekka

AU - Savage, Linda

AU - Harbinson, Jeremy

AU - Kramer, David M.

AU - Merchant, Sabeeha S.

AU - Yeates, Todd

AU - Croce, Roberta

PY - 2018/4/1

Y1 - 2018/4/1

N2 - Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) catalyzes the reaction between gaseous carbon dioxide (CO2) and ribulose-1,5-bisphosphate. Although it is one of the most studied enzymes, the assembly mechanisms of the large hexadecameric RuBisCO is still emerging. In bacteria and in the C4 plant Zea mays, a protein with distant homology to pterin-4α-carbinolamine dehydratase (PCD) has recently been shown to be involved in RuBisCO assembly. However, studies of the homologous PCD-like protein (RAF2, RuBisCO assembly factor 2) in the C3 plant Arabidopsis thaliana (A. thaliana) have so far focused on its role in hormone and stress signaling. We investigated whether A. thalianaRAF2 is also involved in RuBisCO assembly. We localized RAF2 to the soluble chloroplast stroma and demonstrated that raf2 A. thaliana mutant plants display a severe pale green phenotype with reduced levels of stromal RuBisCO. We concluded that the RAF2 protein is probably involved in RuBisCO assembly in the C3 plant A. thaliana.

AB - Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) catalyzes the reaction between gaseous carbon dioxide (CO2) and ribulose-1,5-bisphosphate. Although it is one of the most studied enzymes, the assembly mechanisms of the large hexadecameric RuBisCO is still emerging. In bacteria and in the C4 plant Zea mays, a protein with distant homology to pterin-4α-carbinolamine dehydratase (PCD) has recently been shown to be involved in RuBisCO assembly. However, studies of the homologous PCD-like protein (RAF2, RuBisCO assembly factor 2) in the C3 plant Arabidopsis thaliana (A. thaliana) have so far focused on its role in hormone and stress signaling. We investigated whether A. thalianaRAF2 is also involved in RuBisCO assembly. We localized RAF2 to the soluble chloroplast stroma and demonstrated that raf2 A. thaliana mutant plants display a severe pale green phenotype with reduced levels of stromal RuBisCO. We concluded that the RAF2 protein is probably involved in RuBisCO assembly in the C3 plant A. thaliana.

KW - abscisic acid

KW - Atg5 g51110

KW - chloroplast

KW - RAF2

KW - RuBisCO

KW - RuBisCO aggregation

KW - RuBisCO assembly factor

KW - SDIRIP1

UR - http://www.scopus.com/inward/record.url?scp=85043687702&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85043687702&partnerID=8YFLogxK

U2 - 10.1111/tpj.13849

DO - 10.1111/tpj.13849

M3 - Article

VL - 94

SP - 146

EP - 156

JO - Plant Journal

JF - Plant Journal

SN - 0960-7412

IS - 1

ER -