Radiofrequency tissue ablation in the rabbit lung: Efficacy and complications

S. Nahum Goldberg, G. Scott Gazelle, Carolyn Compton, Theresa C. McLoud

Research output: Contribution to journalArticle

180 Citations (Scopus)

Abstract

Rationale and Objectives.: We assessed the feasibility and safety of performing percutaneous radiofrequency ablation of pulmonary tissue in rabbits. Methods.: Using an aseptic technique and computed tomography (CT) guidance, insulated 19-gauge aspiration biopsy needles were inserted into the right lower lobe of eight New Zealand White rabbits. Radiofrequency was applied via a coaxial electrode for 6 min at 90°C. Probe-tip temperature, tissue impedance, and wattage were recorded at baseline and at 60-sec intervals throughout the procedure. CT scanning was used to assess tissue destruction and the presence or absence of pneumothorax immediately after the procedure and at 24 hr, 3 days, 10 days, 21 days, and 28 days. Three rabbits were sacrificed immediately, and the remaining rabbits were euthanized at 24 hr and at 3 days, 10 days, and 28 days (two rabbits). Gross and microscopic pathology were obtained and correlated with CT findings. Results.: The mean initial tissue impedance was 509 ± 197 Ω; marked changes in tissue impedance were found during the procedure (240-1380 Ω). Rigid temperature control required continuous manual fine-tuning of generator output. Increased respiratory rate was noted in one rabbit during the first 30 sec of radiofrequency application. Homogeneous, ovoid opacities 8.4 ± 2.4 mm in diameter and 1.4 ± 0.1 cm in length were found by CT scanning immediately after the procedure. These opacities showed maximal consolidation at 3 days, corresponding to coagulative necrosis and a peripheral acute inflammatory reaction. At 10 days, peripheral hyperattenuation with central hypoatenuation (early fibrosis surrounding degenerating blood products) was seen. Minimal residual fibrosis, pleural scarring, or both were noted by 28 days, suggesting a rapid, near-total recovery from the procedure. Lesion sizes were within 2 mm of gross pathologic findings. Pneumothoraces were noted in three of the eight rabbits (37.5%). Conclusion.: Radiofrequency tissue ablation was safely performed in pulmonary parenchyma via a percutaneous, transthoracic approach using a coaxial needle technique. Tissue response to thermal injury was predictable and easily monitored by CT scanning with excellent radiologic-pathologic correlation.

Original languageEnglish (US)
Pages (from-to)776-784
Number of pages9
JournalAcademic Radiology
Volume2
Issue number9
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Rabbits
Lung
Tomography
Electric Impedance
Pneumothorax
Needles
Fibrosis
Temperature
Needle Biopsy
Respiratory Rate
Cicatrix
Electrodes
Necrosis
Hot Temperature
Pathology
Safety
Wounds and Injuries

Keywords

  • interventional procedures
  • Lungs
  • radiofrequency
  • tissue ablation

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Radiofrequency tissue ablation in the rabbit lung : Efficacy and complications. / Goldberg, S. Nahum; Gazelle, G. Scott; Compton, Carolyn; McLoud, Theresa C.

In: Academic Radiology, Vol. 2, No. 9, 1995, p. 776-784.

Research output: Contribution to journalArticle

Goldberg, S. Nahum ; Gazelle, G. Scott ; Compton, Carolyn ; McLoud, Theresa C. / Radiofrequency tissue ablation in the rabbit lung : Efficacy and complications. In: Academic Radiology. 1995 ; Vol. 2, No. 9. pp. 776-784.
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AB - Rationale and Objectives.: We assessed the feasibility and safety of performing percutaneous radiofrequency ablation of pulmonary tissue in rabbits. Methods.: Using an aseptic technique and computed tomography (CT) guidance, insulated 19-gauge aspiration biopsy needles were inserted into the right lower lobe of eight New Zealand White rabbits. Radiofrequency was applied via a coaxial electrode for 6 min at 90°C. Probe-tip temperature, tissue impedance, and wattage were recorded at baseline and at 60-sec intervals throughout the procedure. CT scanning was used to assess tissue destruction and the presence or absence of pneumothorax immediately after the procedure and at 24 hr, 3 days, 10 days, 21 days, and 28 days. Three rabbits were sacrificed immediately, and the remaining rabbits were euthanized at 24 hr and at 3 days, 10 days, and 28 days (two rabbits). Gross and microscopic pathology were obtained and correlated with CT findings. Results.: The mean initial tissue impedance was 509 ± 197 Ω; marked changes in tissue impedance were found during the procedure (240-1380 Ω). Rigid temperature control required continuous manual fine-tuning of generator output. Increased respiratory rate was noted in one rabbit during the first 30 sec of radiofrequency application. Homogeneous, ovoid opacities 8.4 ± 2.4 mm in diameter and 1.4 ± 0.1 cm in length were found by CT scanning immediately after the procedure. These opacities showed maximal consolidation at 3 days, corresponding to coagulative necrosis and a peripheral acute inflammatory reaction. At 10 days, peripheral hyperattenuation with central hypoatenuation (early fibrosis surrounding degenerating blood products) was seen. Minimal residual fibrosis, pleural scarring, or both were noted by 28 days, suggesting a rapid, near-total recovery from the procedure. Lesion sizes were within 2 mm of gross pathologic findings. Pneumothoraces were noted in three of the eight rabbits (37.5%). Conclusion.: Radiofrequency tissue ablation was safely performed in pulmonary parenchyma via a percutaneous, transthoracic approach using a coaxial needle technique. Tissue response to thermal injury was predictable and easily monitored by CT scanning with excellent radiologic-pathologic correlation.

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