Radical activated cleavage of peptides and proteins: An alternative to proteolytic digestion

Barbara J. Jones, Laurie E. Locascio, Mark Hayes

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

Radical activated cleavage (RAC) is a new, virtually preparation-free method of protein and peptide fragmentation that produces a distinct pattern in less than 10 s. RAC of peptides uses hydroxyl radical production, at the surface of titanium dioxide (TiO2), to fragment the peptide backbone. Fragmentation of angiotensin I, lys-bradykinin and myoglobin was shown to be reproducible and rapid using RAC. Fragmentation occurred in predictable patterns suggesting cleavage of the peptide bond at proline. Additionally, enkephalin and Peptide A-779, two peptides that do not contain proline, showed no fragmentation under the same conditions. The peptide angiotensin was fragmented using several different device configurations including: (1) small volume wells; (2) microfluidic channels; and (3) a microflow reactor.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages286-288
Number of pages3
ISBN (Print)0974361119, 9780974361116
StatePublished - Jan 1 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: Oct 9 2005Oct 13 2005

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
Country/TerritoryUnited States
CityBoston, MA
Period10/9/0510/13/05

Keywords

  • Cleavage
  • Fragmentation
  • Hydroxyl radical
  • Titanium dioxide

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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