Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser

Lukas Lomb; Thomas R M Barends; Stephan Kassemeyer; Andrew Aquila; Sascha W. Epp; Benjamin Erk; Lutz Foucar; Robert Hartmann; Benedikt Rudek; Daniel Rolles; Artem Rudenko; Robert L. Shoeman; Jakob Andreasson; Sasa Bajt; Miriam Barthelmess; Anton Barty; Michael J. Bogan; Christoph Bostedt; John D. Bozek; Carl Caleman; Ryan Coffee; Nicola Coppola; Daniel P. Deponte; R. Bruce Doak; Tomas Ekeberg; Holger Fleckenstein; Petra Fromme; Maike Gebhardt; Heinz Graafsma; Lars Gumprecht; Christina Y. Hampton; Andreas Hartmann; Günter Hauser; Helmut Hirsemann; Peter Holl; James M. Holton; Mark S. Hunter; Wolfgang Kabsch; Nils Kimmel; Richard Kirian; Mengning Liang; Filipe R N C Maia; Anton Meinhart; Stefano Marchesini; Andrew V. Martin; Karol Nass; Christian Reich; Joachim Schulz; M. Marvin Seibert; Raymond Sierra; Heike Soltau; John Spence; Jan Steinbrener; Francesco Stellato; Stephan Stern; Nicusor Timneanu; Xiaoyu Wang; Georg Weidenspointner; Uwe Weierstall; Thomas A. White; Cornelia Wunderer; Henry N. Chapman; Joachim Ullrich; Lothar Strüder; Ilme Schlichting

doi:10.1103/PhysRevB.84.214111

Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser

Lukas Lomb, Thomas R M Barends, Stephan Kassemeyer, Andrew Aquila, Sascha W. Epp, Benjamin Erk, Lutz Foucar, Robert Hartmann, Benedikt Rudek, Daniel Rolles, Artem Rudenko, Robert L. Shoeman, Jakob Andreasson, Sasa Bajt, Miriam Barthelmess, Anton Barty, Michael J. Bogan, Christoph Bostedt, John D. Bozek, Carl CalemanRyan Coffee, Nicola Coppola, Daniel P. Deponte, R. Bruce Doak, Tomas Ekeberg, Holger Fleckenstein, Petra Fromme, Maike Gebhardt, Heinz Graafsma, Lars Gumprecht, Christina Y. Hampton, Andreas Hartmann, Günter Hauser, Helmut Hirsemann, Peter Holl, James M. Holton, Mark S. Hunter, Wolfgang Kabsch, Nils Kimmel, Richard Kirian, Mengning Liang, Filipe R N C Maia, Anton Meinhart, Stefano Marchesini, Andrew V. Martin, Karol Nass, Christian Reich, Joachim Schulz, M. Marvin Seibert, Raymond Sierra, Heike Soltau, John Spence, Jan Steinbrener, Francesco Stellato, Stephan Stern, Nicusor Timneanu, Xiaoyu Wang, Georg Weidenspointner, Uwe Weierstall, Thomas A. White, Cornelia Wunderer, Henry N. Chapman, Joachim Ullrich, Lothar Strüder, Ilme Schlichting

Research output: Contribution to journal › Article › peer-review

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Abstract

X-ray free-electron lasers deliver intense femtosecond pulses that promise to yield high resolution diffraction data of nanocrystals before the destruction of the sample by radiation damage. Diffraction intensities of lysozyme nanocrystals collected at the Linac Coherent Light Source using 2 keV photons were used for structure determination by molecular replacement and analyzed for radiation damage as a function of pulse length and fluence. Signatures of radiation damage are observed for pulses as short as 70 fs. Parametric scaling used in conventional crystallography does not account for the observed effects.

Original language	English (US)
Article number	214111
Journal	Physical Review B - Condensed Matter and Materials Physics
Volume	84
Issue number	21
DOIs	https://doi.org/10.1103/PhysRevB.84.214111
State	Published - Dec 20 2011

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics

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Lomb, L., Barends, T. R. M., Kassemeyer, S., Aquila, A., Epp, S. W., Erk, B., Foucar, L., Hartmann, R., Rudek, B., Rolles, D., Rudenko, A., Shoeman, R. L., Andreasson, J., Bajt, S., Barthelmess, M., Barty, A., Bogan, M. J., Bostedt, C., Bozek, J. D., ... Schlichting, I. (2011). Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser. Physical Review B - Condensed Matter and Materials Physics, 84(21), Article 214111. https://doi.org/10.1103/PhysRevB.84.214111

Lomb, L, Barends, TRM, Kassemeyer, S, Aquila, A, Epp, SW, Erk, B, Foucar, L, Hartmann, R, Rudek, B, Rolles, D, Rudenko, A, Shoeman, RL, Andreasson, J, Bajt, S, Barthelmess, M, Barty, A, Bogan, MJ, Bostedt, C, Bozek, JD, Caleman, C, Coffee, R, Coppola, N, Deponte, DP, Doak, RB, Ekeberg, T, Fleckenstein, H, Fromme, P, Gebhardt, M, Graafsma, H, Gumprecht, L, Hampton, CY, Hartmann, A, Hauser, G, Hirsemann, H, Holl, P, Holton, JM, Hunter, MS, Kabsch, W, Kimmel, N, Kirian, R, Liang, M, Maia, FRNC, Meinhart, A, Marchesini, S, Martin, AV, Nass, K, Reich, C, Schulz, J, Seibert, MM, Sierra, R, Soltau, H, Spence, J, Steinbrener, J, Stellato, F, Stern, S, Timneanu, N, Wang, X, Weidenspointner, G, Weierstall, U, White, TA, Wunderer, C, Chapman, HN, Ullrich, J, Strüder, L & Schlichting, I 2011, 'Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser', Physical Review B - Condensed Matter and Materials Physics, vol. 84, no. 21, 214111. https://doi.org/10.1103/PhysRevB.84.214111

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title = "Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser",

abstract = "X-ray free-electron lasers deliver intense femtosecond pulses that promise to yield high resolution diffraction data of nanocrystals before the destruction of the sample by radiation damage. Diffraction intensities of lysozyme nanocrystals collected at the Linac Coherent Light Source using 2 keV photons were used for structure determination by molecular replacement and analyzed for radiation damage as a function of pulse length and fluence. Signatures of radiation damage are observed for pulses as short as 70 fs. Parametric scaling used in conventional crystallography does not account for the observed effects.",

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AU - Lomb, Lukas

AU - Barends, Thomas R M

AU - Kassemeyer, Stephan

AU - Aquila, Andrew

AU - Epp, Sascha W.

AU - Erk, Benjamin

AU - Foucar, Lutz

AU - Hartmann, Robert

AU - Rudek, Benedikt

AU - Rolles, Daniel

AU - Rudenko, Artem

AU - Shoeman, Robert L.

AU - Andreasson, Jakob

AU - Bajt, Sasa

AU - Barthelmess, Miriam

AU - Barty, Anton

AU - Bogan, Michael J.

AU - Bostedt, Christoph

AU - Bozek, John D.

AU - Caleman, Carl

AU - Coffee, Ryan

AU - Coppola, Nicola

AU - Deponte, Daniel P.

AU - Doak, R. Bruce

AU - Ekeberg, Tomas

AU - Fleckenstein, Holger

AU - Fromme, Petra

AU - Gebhardt, Maike

AU - Graafsma, Heinz

AU - Gumprecht, Lars

AU - Hampton, Christina Y.

AU - Hartmann, Andreas

AU - Hauser, Günter

AU - Hirsemann, Helmut

AU - Holl, Peter

AU - Holton, James M.

AU - Hunter, Mark S.

AU - Kabsch, Wolfgang

AU - Kimmel, Nils

AU - Kirian, Richard

AU - Liang, Mengning

AU - Maia, Filipe R N C

AU - Meinhart, Anton

AU - Marchesini, Stefano

AU - Martin, Andrew V.

AU - Nass, Karol

AU - Reich, Christian

AU - Schulz, Joachim

AU - Seibert, M. Marvin

AU - Sierra, Raymond

AU - Soltau, Heike

AU - Spence, John

AU - Steinbrener, Jan

AU - Stellato, Francesco

AU - Stern, Stephan

AU - Timneanu, Nicusor

AU - Wang, Xiaoyu

AU - Weidenspointner, Georg

AU - Weierstall, Uwe

AU - White, Thomas A.

AU - Wunderer, Cornelia

AU - Chapman, Henry N.

AU - Ullrich, Joachim

AU - Strüder, Lothar

AU - Schlichting, Ilme

PY - 2011/12/20

Y1 - 2011/12/20

N2 - X-ray free-electron lasers deliver intense femtosecond pulses that promise to yield high resolution diffraction data of nanocrystals before the destruction of the sample by radiation damage. Diffraction intensities of lysozyme nanocrystals collected at the Linac Coherent Light Source using 2 keV photons were used for structure determination by molecular replacement and analyzed for radiation damage as a function of pulse length and fluence. Signatures of radiation damage are observed for pulses as short as 70 fs. Parametric scaling used in conventional crystallography does not account for the observed effects.

AB - X-ray free-electron lasers deliver intense femtosecond pulses that promise to yield high resolution diffraction data of nanocrystals before the destruction of the sample by radiation damage. Diffraction intensities of lysozyme nanocrystals collected at the Linac Coherent Light Source using 2 keV photons were used for structure determination by molecular replacement and analyzed for radiation damage as a function of pulse length and fluence. Signatures of radiation damage are observed for pulses as short as 70 fs. Parametric scaling used in conventional crystallography does not account for the observed effects.

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DO - 10.1103/PhysRevB.84.214111

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SN - 1098-0121

VL - 84

JO - Physical Review B - Condensed Matter and Materials Physics

JF - Physical Review B - Condensed Matter and Materials Physics

IS - 21

M1 - 214111

ER -

Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser

Abstract

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