Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser

Lukas Lomb, Thomas R M Barends, Stephan Kassemeyer, Andrew Aquila, Sascha W. Epp, Benjamin Erk, Lutz Foucar, Robert Hartmann, Benedikt Rudek, Daniel Rolles, Artem Rudenko, Robert L. Shoeman, Jakob Andreasson, Sasa Bajt, Miriam Barthelmess, Anton Barty, Michael J. Bogan, Christoph Bostedt, John D. Bozek, Carl CalemanRyan Coffee, Nicola Coppola, Daniel P. Deponte, R. Bruce Doak, Tomas Ekeberg, Holger Fleckenstein, Petra Fromme, Maike Gebhardt, Heinz Graafsma, Lars Gumprecht, Christina Y. Hampton, Andreas Hartmann, Günter Hauser, Helmut Hirsemann, Peter Holl, James M. Holton, Mark S. Hunter, Wolfgang Kabsch, Nils Kimmel, Richard Kirian, Mengning Liang, Filipe R N C Maia, Anton Meinhart, Stefano Marchesini, Andrew V. Martin, Karol Nass, Christian Reich, Joachim Schulz, M. Marvin Seibert, Raymond Sierra, Heike Soltau, John Spence, Jan Steinbrener, Francesco Stellato, Stephan Stern, Nicusor Timneanu, Xiaoyu Wang, Georg Weidenspointner, Uwe Weierstall, Thomas A. White, Cornelia Wunderer, Henry N. Chapman, Joachim Ullrich, Lothar Strüder, Ilme Schlichting

Research output: Contribution to journalArticle

124 Scopus citations

Abstract

X-ray free-electron lasers deliver intense femtosecond pulses that promise to yield high resolution diffraction data of nanocrystals before the destruction of the sample by radiation damage. Diffraction intensities of lysozyme nanocrystals collected at the Linac Coherent Light Source using 2 keV photons were used for structure determination by molecular replacement and analyzed for radiation damage as a function of pulse length and fluence. Signatures of radiation damage are observed for pulses as short as 70 fs. Parametric scaling used in conventional crystallography does not account for the observed effects.

Original languageEnglish (US)
Article number214111
JournalPhysical Review B - Condensed Matter and Materials Physics
Volume84
Issue number21
DOIs
StatePublished - Dec 20 2011

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ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Lomb, L., Barends, T. R. M., Kassemeyer, S., Aquila, A., Epp, S. W., Erk, B., Foucar, L., Hartmann, R., Rudek, B., Rolles, D., Rudenko, A., Shoeman, R. L., Andreasson, J., Bajt, S., Barthelmess, M., Barty, A., Bogan, M. J., Bostedt, C., Bozek, J. D., ... Schlichting, I. (2011). Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser. Physical Review B - Condensed Matter and Materials Physics, 84(21), [214111]. https://doi.org/10.1103/PhysRevB.84.214111