Quick-freeze, deep-etch, rotary-shadow views of the extracellular matrix and cortical cytoskeleton of Xenopus laevis eggs

C. A. Larabell, D. E. Chandler

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The quick-freeze, deep-etch, rotary-shadow technique provides a powerful tool to study the structural dynamics of extracellular matrices. Using this technique, we show that the extracellular investments of the Xenopus laevis egg are multilayered and securely anchored to the egg surface. The cortical cytoskeleton within the egg contains embedded cortical granules with surrounding endoplasmic reticulum and is capped by a thin reticular sheet that contacts the inner surface of the plasma membrane. The extracellular matrix undergoes three distinct changes at fertilization: a) formation of a 'smooth' layer below the vitelline envelope (VE), b) transformation of the VE itself to an altered VE composed of concentric fibrous sheets, and c) formation of a dense, 'briar-patch'-like fertilization layer at the upper surface of the VE.

Original languageEnglish (US)
Pages (from-to)228-243
Number of pages16
JournalJournal of Electron Microscopy Technique
Volume13
Issue number3
StatePublished - 1989

Fingerprint

eggs
Xenopus laevis
Cytoskeleton
Eggs
Extracellular Matrix
Ovum
envelopes
fertilization
Fertilization
matrices
Structural dynamics
Cell membranes
endoplasmic reticulum
Endoplasmic Reticulum
dynamic structural analysis
Cell Membrane
membranes

Keywords

  • Electron microscopy
  • Fertilization
  • Freeze-fracture

ASJC Scopus subject areas

  • Anatomy

Cite this

Quick-freeze, deep-etch, rotary-shadow views of the extracellular matrix and cortical cytoskeleton of Xenopus laevis eggs. / Larabell, C. A.; Chandler, D. E.

In: Journal of Electron Microscopy Technique, Vol. 13, No. 3, 1989, p. 228-243.

Research output: Contribution to journalArticle

@article{4c02dc377e7740cfb8bc39bbde7f20ad,
title = "Quick-freeze, deep-etch, rotary-shadow views of the extracellular matrix and cortical cytoskeleton of Xenopus laevis eggs",
abstract = "The quick-freeze, deep-etch, rotary-shadow technique provides a powerful tool to study the structural dynamics of extracellular matrices. Using this technique, we show that the extracellular investments of the Xenopus laevis egg are multilayered and securely anchored to the egg surface. The cortical cytoskeleton within the egg contains embedded cortical granules with surrounding endoplasmic reticulum and is capped by a thin reticular sheet that contacts the inner surface of the plasma membrane. The extracellular matrix undergoes three distinct changes at fertilization: a) formation of a 'smooth' layer below the vitelline envelope (VE), b) transformation of the VE itself to an altered VE composed of concentric fibrous sheets, and c) formation of a dense, 'briar-patch'-like fertilization layer at the upper surface of the VE.",
keywords = "Electron microscopy, Fertilization, Freeze-fracture",
author = "Larabell, {C. A.} and Chandler, {D. E.}",
year = "1989",
language = "English (US)",
volume = "13",
pages = "228--243",
journal = "Microscopy Research and Technique",
issn = "1059-910X",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Quick-freeze, deep-etch, rotary-shadow views of the extracellular matrix and cortical cytoskeleton of Xenopus laevis eggs

AU - Larabell, C. A.

AU - Chandler, D. E.

PY - 1989

Y1 - 1989

N2 - The quick-freeze, deep-etch, rotary-shadow technique provides a powerful tool to study the structural dynamics of extracellular matrices. Using this technique, we show that the extracellular investments of the Xenopus laevis egg are multilayered and securely anchored to the egg surface. The cortical cytoskeleton within the egg contains embedded cortical granules with surrounding endoplasmic reticulum and is capped by a thin reticular sheet that contacts the inner surface of the plasma membrane. The extracellular matrix undergoes three distinct changes at fertilization: a) formation of a 'smooth' layer below the vitelline envelope (VE), b) transformation of the VE itself to an altered VE composed of concentric fibrous sheets, and c) formation of a dense, 'briar-patch'-like fertilization layer at the upper surface of the VE.

AB - The quick-freeze, deep-etch, rotary-shadow technique provides a powerful tool to study the structural dynamics of extracellular matrices. Using this technique, we show that the extracellular investments of the Xenopus laevis egg are multilayered and securely anchored to the egg surface. The cortical cytoskeleton within the egg contains embedded cortical granules with surrounding endoplasmic reticulum and is capped by a thin reticular sheet that contacts the inner surface of the plasma membrane. The extracellular matrix undergoes three distinct changes at fertilization: a) formation of a 'smooth' layer below the vitelline envelope (VE), b) transformation of the VE itself to an altered VE composed of concentric fibrous sheets, and c) formation of a dense, 'briar-patch'-like fertilization layer at the upper surface of the VE.

KW - Electron microscopy

KW - Fertilization

KW - Freeze-fracture

UR - http://www.scopus.com/inward/record.url?scp=0024460003&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024460003&partnerID=8YFLogxK

M3 - Article

VL - 13

SP - 228

EP - 243

JO - Microscopy Research and Technique

JF - Microscopy Research and Technique

SN - 1059-910X

IS - 3

ER -