Queen conch (Strombus gigas) testis regresses during the reproductive season at nearshore sites in the florida keys

Daniel J. Spade, Robert J. Griffitt, Li Liu, Nancy J. Brown-Peterson, Kevin J. Kroll, April Feswick, Obert A. Glazer, David S. Barber, Nancy D. Denslow

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: Queen conch (Strombus gigas) reproduction is inhibited in nearshore areas of the Florida Keys, relative to the offshore environment where conchs reproduce successfully. Nearshore reproductive failure is possibly a result of exposure to environmental factors, including heavy metals, which are likely to accumulate close to shore. Metals such as Cu and Zn are detrimental to reproduction in many mollusks. Methodology/Principal Findings: Histology shows gonadal atrophy in nearshore conchs as compared to reproductively healthy offshore conchs. In order to determine molecular mechanisms leading to tissue changes and reproductive failure, a microarray was developed. A normalized cDNA library for queen conch was constructed and sequenced using the 454 Life Sciences GS-FLX pyrosequencer, producing 27,723 assembled contigs and 7,740 annotated transcript sequences. The resulting sequences were used to design the microarray. Microarray analysis of conch testis indicated differential regulation of 255 genes (p

Original languageEnglish (US)
Article numbere12737
Pages (from-to)1-14
Number of pages14
JournalPLoS One
Volume5
Issue number9
DOIs
StatePublished - 2010
Externally publishedYes

Fingerprint

Microarrays
Reproduction
Testis
breeding season
testes
Biological Science Disciplines
Mollusca
Environmental Exposure
Microarray Analysis
Heavy Metals
Gene Library
Atrophy
Histology
Metals
atrophy
cDNA libraries
histology
molluscs
heavy metals
Genes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Spade, D. J., Griffitt, R. J., Liu, L., Brown-Peterson, N. J., Kroll, K. J., Feswick, A., ... Denslow, N. D. (2010). Queen conch (Strombus gigas) testis regresses during the reproductive season at nearshore sites in the florida keys. PLoS One, 5(9), 1-14. [e12737]. https://doi.org/10.1371/journal.pone.0012737

Queen conch (Strombus gigas) testis regresses during the reproductive season at nearshore sites in the florida keys. / Spade, Daniel J.; Griffitt, Robert J.; Liu, Li; Brown-Peterson, Nancy J.; Kroll, Kevin J.; Feswick, April; Glazer, Obert A.; Barber, David S.; Denslow, Nancy D.

In: PLoS One, Vol. 5, No. 9, e12737, 2010, p. 1-14.

Research output: Contribution to journalArticle

Spade, DJ, Griffitt, RJ, Liu, L, Brown-Peterson, NJ, Kroll, KJ, Feswick, A, Glazer, OA, Barber, DS & Denslow, ND 2010, 'Queen conch (Strombus gigas) testis regresses during the reproductive season at nearshore sites in the florida keys', PLoS One, vol. 5, no. 9, e12737, pp. 1-14. https://doi.org/10.1371/journal.pone.0012737
Spade, Daniel J. ; Griffitt, Robert J. ; Liu, Li ; Brown-Peterson, Nancy J. ; Kroll, Kevin J. ; Feswick, April ; Glazer, Obert A. ; Barber, David S. ; Denslow, Nancy D. / Queen conch (Strombus gigas) testis regresses during the reproductive season at nearshore sites in the florida keys. In: PLoS One. 2010 ; Vol. 5, No. 9. pp. 1-14.
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