Quantitative high-throughput analysis of synthetic genetic interactions in Caenorhabditis elegans by RNA interference

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Biological processes are highly dynamic but the current representation of molecular networks is static and largely qualitative. To investigate the dynamic property of genetic networks, a novel quantitative high-throughput method based on RNA interference and capable of calculating the relevance of each interaction, was developed. With this approach, it will be possible to identify not only the components of a network, but also to investigate quantitatively how network and biological processes react to perturbations. As a first application of this method, the genetic interactions of a weak loss-of-function mutation in the gene efl-1/E2F with all the genes of chromosome III were investigated during embryonic development of Caenorhabditis elegans. Fifteen synthetic genetic interactions of efl-1/E2F with the genes of chromosome III were detected, measured and ranked by statistical relevance.

Original languageEnglish (US)
Pages (from-to)392-396
Number of pages5
JournalGenomics
Volume93
Issue number4
DOIs
StatePublished - Apr 1 2009
Externally publishedYes

Fingerprint

Caenorhabditis elegans
RNA Interference
Biological Phenomena
Chromosomes
Genes
Embryonic Development
Mutation

Keywords

  • Caenorhabditis elegans
  • Chromosome III
  • Efl-1/E2F
  • Embryonic development
  • Interference of RNA
  • Synthetic phenotype

ASJC Scopus subject areas

  • Genetics

Cite this

Quantitative high-throughput analysis of synthetic genetic interactions in Caenorhabditis elegans by RNA interference. / Fortunato, Angelo.

In: Genomics, Vol. 93, No. 4, 01.04.2009, p. 392-396.

Research output: Contribution to journalArticle

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