Quantifying Condition-Dependent Intracellular Protein Levels Enables High-Precision Fitness Estimates

Kerry A. Geiler-Samerotte, Tatsunori Hashimoto, Michael F. Dion, Bogdan A. Budnik, Edoardo M. Airoldi, D. Allan Drummond

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Countless studies monitor the growth rate of microbial populations as a measure of fitness. However, an enormous gap separates growth-rate differences measurable in the laboratory from those that natural selection can distinguish efficiently. Taking advantage of the recent discovery that transcript and protein levels in budding yeast closely track growth rate, we explore the possibility that growth rate can be more sensitively inferred by monitoring the proteomic response to growth, rather than growth itself. We find a set of proteins whose levels, in aggregate, enable prediction of growth rate to a higher precision than direct measurements. However, we find little overlap between these proteins and those that closely track growth rate in other studies. These results suggest that, in yeast, the pathways that set the pace of cell division can differ depending on the growth-altering stimulus. Still, with proper validation, protein measurements can provide high-precision growth estimates that allow extension of phenotypic growth-based assays closer to the limits of evolutionary selection.

Original languageEnglish (US)
Article numbere75320
JournalPloS one
Volume8
Issue number9
DOIs
StatePublished - Sep 25 2013
Externally publishedYes

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Growth
Proteins
proteins
yeasts
Yeast
monitoring
protein aggregates
proteomics
natural selection
cell division
prediction
Assays
assays
Cells
Saccharomycetales
Genetic Selection
Monitoring
Cell Division
Proteomics
Yeasts

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Quantifying Condition-Dependent Intracellular Protein Levels Enables High-Precision Fitness Estimates. / Geiler-Samerotte, Kerry A.; Hashimoto, Tatsunori; Dion, Michael F.; Budnik, Bogdan A.; Airoldi, Edoardo M.; Drummond, D. Allan.

In: PloS one, Vol. 8, No. 9, e75320, 25.09.2013.

Research output: Contribution to journalArticle

Geiler-Samerotte, Kerry A. ; Hashimoto, Tatsunori ; Dion, Michael F. ; Budnik, Bogdan A. ; Airoldi, Edoardo M. ; Drummond, D. Allan. / Quantifying Condition-Dependent Intracellular Protein Levels Enables High-Precision Fitness Estimates. In: PloS one. 2013 ; Vol. 8, No. 9.
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