Quantifying antibody binding on protein microarrays using microarray nonlinear calibration

Xiaobo Yu, Garrick Wallstrom, Dewey Magee, D. Eliseo A Mendoza, Jie Wang, Xiaofang Bian, Morgan Graves, Joshua LaBaer

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We present a microarray nonlinear calibration (MiNC) method for quantifying antibody binding to the surface of protein microarrays that significantly increases the linear dynamic range and reduces assay variation compared with traditional approaches. A serological analysis of guinea pig Mycobacterium tuberculosis models showed that a larger number of putative antigen targets were identified with MiNC, which is consistent with the improved assay performance of protein microarrays. MiNC has the potential to be employed in biomedical research using multiplex antibody assays that need quantitation, including the discovery of antibody biomarkers, clinical diagnostics with multi-antibody signatures, and construction of immune mathematical models.

Original languageEnglish (US)
Pages (from-to)257-264
Number of pages8
JournalBioTechniques
Volume54
Issue number5
DOIs
StatePublished - May 2013

Fingerprint

Protein Array Analysis
Microarrays
Calibration
Carrier Proteins
Antibodies
Assays
Proteins
Mycobacterium tuberculosis
Biomedical Research
Biomarkers
Guinea Pigs
Membrane Proteins
Theoretical Models
Antigens
Mathematical models

Keywords

  • Microarray nonlinear calibration
  • Nn vitro transcription/translation
  • Nucleic acid programmable protein arrays
  • Tuberculosis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biotechnology

Cite this

Quantifying antibody binding on protein microarrays using microarray nonlinear calibration. / Yu, Xiaobo; Wallstrom, Garrick; Magee, Dewey; Mendoza, D. Eliseo A; Wang, Jie; Bian, Xiaofang; Graves, Morgan; LaBaer, Joshua.

In: BioTechniques, Vol. 54, No. 5, 05.2013, p. 257-264.

Research output: Contribution to journalArticle

Yu, Xiaobo ; Wallstrom, Garrick ; Magee, Dewey ; Mendoza, D. Eliseo A ; Wang, Jie ; Bian, Xiaofang ; Graves, Morgan ; LaBaer, Joshua. / Quantifying antibody binding on protein microarrays using microarray nonlinear calibration. In: BioTechniques. 2013 ; Vol. 54, No. 5. pp. 257-264.
@article{97faa281111544de961fdf1c6c32088a,
title = "Quantifying antibody binding on protein microarrays using microarray nonlinear calibration",
abstract = "We present a microarray nonlinear calibration (MiNC) method for quantifying antibody binding to the surface of protein microarrays that significantly increases the linear dynamic range and reduces assay variation compared with traditional approaches. A serological analysis of guinea pig Mycobacterium tuberculosis models showed that a larger number of putative antigen targets were identified with MiNC, which is consistent with the improved assay performance of protein microarrays. MiNC has the potential to be employed in biomedical research using multiplex antibody assays that need quantitation, including the discovery of antibody biomarkers, clinical diagnostics with multi-antibody signatures, and construction of immune mathematical models.",
keywords = "Microarray nonlinear calibration, Nn vitro transcription/translation, Nucleic acid programmable protein arrays, Tuberculosis",
author = "Xiaobo Yu and Garrick Wallstrom and Dewey Magee and Mendoza, {D. Eliseo A} and Jie Wang and Xiaofang Bian and Morgan Graves and Joshua LaBaer",
year = "2013",
month = "5",
doi = "10.2144/000114028",
language = "English (US)",
volume = "54",
pages = "257--264",
journal = "BioTechniques",
issn = "0736-6205",
publisher = "Eaton Publishing Company",
number = "5",

}

TY - JOUR

T1 - Quantifying antibody binding on protein microarrays using microarray nonlinear calibration

AU - Yu, Xiaobo

AU - Wallstrom, Garrick

AU - Magee, Dewey

AU - Mendoza, D. Eliseo A

AU - Wang, Jie

AU - Bian, Xiaofang

AU - Graves, Morgan

AU - LaBaer, Joshua

PY - 2013/5

Y1 - 2013/5

N2 - We present a microarray nonlinear calibration (MiNC) method for quantifying antibody binding to the surface of protein microarrays that significantly increases the linear dynamic range and reduces assay variation compared with traditional approaches. A serological analysis of guinea pig Mycobacterium tuberculosis models showed that a larger number of putative antigen targets were identified with MiNC, which is consistent with the improved assay performance of protein microarrays. MiNC has the potential to be employed in biomedical research using multiplex antibody assays that need quantitation, including the discovery of antibody biomarkers, clinical diagnostics with multi-antibody signatures, and construction of immune mathematical models.

AB - We present a microarray nonlinear calibration (MiNC) method for quantifying antibody binding to the surface of protein microarrays that significantly increases the linear dynamic range and reduces assay variation compared with traditional approaches. A serological analysis of guinea pig Mycobacterium tuberculosis models showed that a larger number of putative antigen targets were identified with MiNC, which is consistent with the improved assay performance of protein microarrays. MiNC has the potential to be employed in biomedical research using multiplex antibody assays that need quantitation, including the discovery of antibody biomarkers, clinical diagnostics with multi-antibody signatures, and construction of immune mathematical models.

KW - Microarray nonlinear calibration

KW - Nn vitro transcription/translation

KW - Nucleic acid programmable protein arrays

KW - Tuberculosis

UR - http://www.scopus.com/inward/record.url?scp=84877872926&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84877872926&partnerID=8YFLogxK

U2 - 10.2144/000114028

DO - 10.2144/000114028

M3 - Article

C2 - 23662896

AN - SCOPUS:84877872926

VL - 54

SP - 257

EP - 264

JO - BioTechniques

JF - BioTechniques

SN - 0736-6205

IS - 5

ER -