Quantifying antibody binding on protein microarrays using microarray nonlinear calibration

Xiaobo Yu, Garrick Wallstrom, Dewey Magee, D. Eliseo A Mendoza, Jie Wang, Xiaofang Bian, Morgan Graves, Joshua LaBaer

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

We present a microarray nonlinear calibration (MiNC) method for quantifying antibody binding to the surface of protein microarrays that significantly increases the linear dynamic range and reduces assay variation compared with traditional approaches. A serological analysis of guinea pig Mycobacterium tuberculosis models showed that a larger number of putative antigen targets were identified with MiNC, which is consistent with the improved assay performance of protein microarrays. MiNC has the potential to be employed in biomedical research using multiplex antibody assays that need quantitation, including the discovery of antibody biomarkers, clinical diagnostics with multi-antibody signatures, and construction of immune mathematical models.

Original languageEnglish (US)
Pages (from-to)257-264
Number of pages8
JournalBioTechniques
Volume54
Issue number5
DOIs
StatePublished - May 1 2013

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Keywords

  • Microarray nonlinear calibration
  • Nn vitro transcription/translation
  • Nucleic acid programmable protein arrays
  • Tuberculosis

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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