Abstract

A biofilm anode acclimated with growth media containing acetate, then acetate + methane, and finally methane alone produced electrical current in a microbial electrochemical cell (MxC) fed with methane as the sole electron donor. Geobacter was the dominant genus for the bacterial domain (93%) in the biofilm anode, while methanogens (Methanocorpusculum labreanum and Methanosaeta concilii) accounted for 82% of the total archaeal clones in the biofilm. Fluorescence in situ hybridization (FISH) imaging clearly showed a biofilm of mixed bacteria and archaea, suggesting a syntrophic interaction between them for performing anaerobic oxidation of methane (AOM) in the biofilm anode. Measured cumulative coulombs were linearly correlated to the methane-gas concentration in the range of 10–99.97% (R2 ≥ 0.99) when the measurement was sustained for at least 50 min Thus, cumulative coulombs over 50 min could be used to quantify the methane concentration in gas samples.

Original languageEnglish (US)
Pages (from-to)979-984
Number of pages6
JournalBioresource Technology
Volume241
DOIs
StatePublished - Oct 1 2017

Fingerprint

Methane
Biofilms
methane
biofilm
oxidation
electron
Oxidation
Electrons
Anodes
acetate
Acetates
Gases
Methanogens
Electrochemical cells
gas
clone
Bacteria
fluorescence
Fluorescence
Imaging techniques

Keywords

  • Anaerobic oxidation of methane
  • Extracellular electron transfer
  • Methane concentration
  • Reverse methanogenesis
  • Sensors

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Quantification of the methane concentration using anaerobic oxidation of methane coupled to extracellular electron transfer. / Gao, Yaohuan; Ryu, Hodon; Rittmann, Bruce; Hussain, Abid; Lee, Hyung Sool.

In: Bioresource Technology, Vol. 241, 01.10.2017, p. 979-984.

Research output: Contribution to journalArticle

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AU - Gao, Yaohuan

AU - Ryu, Hodon

AU - Rittmann, Bruce

AU - Hussain, Abid

AU - Lee, Hyung Sool

PY - 2017/10/1

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N2 - A biofilm anode acclimated with growth media containing acetate, then acetate + methane, and finally methane alone produced electrical current in a microbial electrochemical cell (MxC) fed with methane as the sole electron donor. Geobacter was the dominant genus for the bacterial domain (93%) in the biofilm anode, while methanogens (Methanocorpusculum labreanum and Methanosaeta concilii) accounted for 82% of the total archaeal clones in the biofilm. Fluorescence in situ hybridization (FISH) imaging clearly showed a biofilm of mixed bacteria and archaea, suggesting a syntrophic interaction between them for performing anaerobic oxidation of methane (AOM) in the biofilm anode. Measured cumulative coulombs were linearly correlated to the methane-gas concentration in the range of 10–99.97% (R2 ≥ 0.99) when the measurement was sustained for at least 50 min Thus, cumulative coulombs over 50 min could be used to quantify the methane concentration in gas samples.

AB - A biofilm anode acclimated with growth media containing acetate, then acetate + methane, and finally methane alone produced electrical current in a microbial electrochemical cell (MxC) fed with methane as the sole electron donor. Geobacter was the dominant genus for the bacterial domain (93%) in the biofilm anode, while methanogens (Methanocorpusculum labreanum and Methanosaeta concilii) accounted for 82% of the total archaeal clones in the biofilm. Fluorescence in situ hybridization (FISH) imaging clearly showed a biofilm of mixed bacteria and archaea, suggesting a syntrophic interaction between them for performing anaerobic oxidation of methane (AOM) in the biofilm anode. Measured cumulative coulombs were linearly correlated to the methane-gas concentration in the range of 10–99.97% (R2 ≥ 0.99) when the measurement was sustained for at least 50 min Thus, cumulative coulombs over 50 min could be used to quantify the methane concentration in gas samples.

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