Quantification of the effect of substrate concentration on the conjugal transfer rate of the TOL plasmid in short‐term batch mating experiments

B. F. Smets, B. E. Rittmann, D. A. Stahl

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Batch mating experiments with Pseudomonas putida PAW 1 (TOL) as a donor and Pseudomonas aeruginosa PAO 1162 as a recipient strain were performed to quantify the effect of the substrate concentration in the mating medium on the observed plasmid transfer rate coefficient. The impact of the substrate concentration in the mating medium was highly correlated with the growth history of the donor strain. When the donor strain was harvested in exponential growth phase, no impact was observed; when the donor strain was taken from the stationary phase, however, a strong impact of the substrate concentration was measured: a 10‐fold reduction in the substrate concentration decreased the observed plasmid transfer rate by 55%.

Original languageEnglish (US)
Pages (from-to)167-172
Number of pages6
JournalLetters in Applied Microbiology
Volume21
Issue number3
DOIs
StatePublished - Sep 1995
Externally publishedYes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Quantification of the effect of substrate concentration on the conjugal transfer rate of the TOL plasmid in short‐term batch mating experiments'. Together they form a unique fingerprint.

  • Cite this