Quantification of methanogenic groups in anaerobic biological reactors by oligonucleotide probe hybridization

L. Raskin, L. K. Poulsen, D. R. Noguera, B. E. Rittmann, D. A. Stahl

Research output: Contribution to journalArticle

288 Scopus citations

Abstract

The microbial community structure of anaerobic biological reactors was evaluated by using oligonucleotide probes complementary to conserved tracts of the 16S rRNAs of phylogenetically defined groups of methanogens. Phylogenetically defined groups of methanogens were quantified and visualized, respectively, by hybridization of 32P- and fluorescent-dye- labeled probes to the 16S rRNAs from samples taken from laboratory acetate- fed chemostats, laboratory municipal solid waste digestors, and full-scale sewage sludge digestors. Methanosarcina species, members of the order Methanobacteriales, and Methanosaeta species were the most abundant methanogens present in the chemostats, the solid-waste digestors, and the sewage sludge digestors, respectively.

Original languageEnglish (US)
Pages (from-to)1241-1248
Number of pages8
JournalApplied and environmental microbiology
Volume60
Issue number4
DOIs
StatePublished - 1994
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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