Quantification of methanogenic groups in anaerobic biological reactors by oligonucleotide probe hybridization

L. Raskin, L. K. Poulsen, D. R. Noguera, Bruce Rittmann, D. A. Stahl

Research output: Contribution to journalArticle

288 Citations (Scopus)

Abstract

The microbial community structure of anaerobic biological reactors was evaluated by using oligonucleotide probes complementary to conserved tracts of the 16S rRNAs of phylogenetically defined groups of methanogens. Phylogenetically defined groups of methanogens were quantified and visualized, respectively, by hybridization of 32P- and fluorescent-dye- labeled probes to the 16S rRNAs from samples taken from laboratory acetate- fed chemostats, laboratory municipal solid waste digestors, and full-scale sewage sludge digestors. Methanosarcina species, members of the order Methanobacteriales, and Methanosaeta species were the most abundant methanogens present in the chemostats, the solid-waste digestors, and the sewage sludge digestors, respectively.

Original languageEnglish (US)
Pages (from-to)1241-1248
Number of pages8
JournalApplied and Environmental Microbiology
Volume60
Issue number4
StatePublished - 1994
Externally publishedYes

Fingerprint

chemostat
oligonucleotide probes
methanogens
Oligonucleotide Probes
Sewage
hybridization
probe
sewage sludge
Solid Waste
Methanobacteriales
Methanosaeta
Methanosarcina
ribosomal RNA
municipal solid waste
solid waste
microbial community
acetate
dye
community structure
solid wastes

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Science(all)
  • Microbiology
  • Earth and Planetary Sciences(all)

Cite this

Quantification of methanogenic groups in anaerobic biological reactors by oligonucleotide probe hybridization. / Raskin, L.; Poulsen, L. K.; Noguera, D. R.; Rittmann, Bruce; Stahl, D. A.

In: Applied and Environmental Microbiology, Vol. 60, No. 4, 1994, p. 1241-1248.

Research output: Contribution to journalArticle

@article{205926dbfd8b402695a3150c0d51d504,
title = "Quantification of methanogenic groups in anaerobic biological reactors by oligonucleotide probe hybridization",
abstract = "The microbial community structure of anaerobic biological reactors was evaluated by using oligonucleotide probes complementary to conserved tracts of the 16S rRNAs of phylogenetically defined groups of methanogens. Phylogenetically defined groups of methanogens were quantified and visualized, respectively, by hybridization of 32P- and fluorescent-dye- labeled probes to the 16S rRNAs from samples taken from laboratory acetate- fed chemostats, laboratory municipal solid waste digestors, and full-scale sewage sludge digestors. Methanosarcina species, members of the order Methanobacteriales, and Methanosaeta species were the most abundant methanogens present in the chemostats, the solid-waste digestors, and the sewage sludge digestors, respectively.",
author = "L. Raskin and Poulsen, {L. K.} and Noguera, {D. R.} and Bruce Rittmann and Stahl, {D. A.}",
year = "1994",
language = "English (US)",
volume = "60",
pages = "1241--1248",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "4",

}

TY - JOUR

T1 - Quantification of methanogenic groups in anaerobic biological reactors by oligonucleotide probe hybridization

AU - Raskin, L.

AU - Poulsen, L. K.

AU - Noguera, D. R.

AU - Rittmann, Bruce

AU - Stahl, D. A.

PY - 1994

Y1 - 1994

N2 - The microbial community structure of anaerobic biological reactors was evaluated by using oligonucleotide probes complementary to conserved tracts of the 16S rRNAs of phylogenetically defined groups of methanogens. Phylogenetically defined groups of methanogens were quantified and visualized, respectively, by hybridization of 32P- and fluorescent-dye- labeled probes to the 16S rRNAs from samples taken from laboratory acetate- fed chemostats, laboratory municipal solid waste digestors, and full-scale sewage sludge digestors. Methanosarcina species, members of the order Methanobacteriales, and Methanosaeta species were the most abundant methanogens present in the chemostats, the solid-waste digestors, and the sewage sludge digestors, respectively.

AB - The microbial community structure of anaerobic biological reactors was evaluated by using oligonucleotide probes complementary to conserved tracts of the 16S rRNAs of phylogenetically defined groups of methanogens. Phylogenetically defined groups of methanogens were quantified and visualized, respectively, by hybridization of 32P- and fluorescent-dye- labeled probes to the 16S rRNAs from samples taken from laboratory acetate- fed chemostats, laboratory municipal solid waste digestors, and full-scale sewage sludge digestors. Methanosarcina species, members of the order Methanobacteriales, and Methanosaeta species were the most abundant methanogens present in the chemostats, the solid-waste digestors, and the sewage sludge digestors, respectively.

UR - http://www.scopus.com/inward/record.url?scp=0028165833&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028165833&partnerID=8YFLogxK

M3 - Article

C2 - 7517129

AN - SCOPUS:0028165833

VL - 60

SP - 1241

EP - 1248

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 4

ER -