QA Binding to D2 Contributes to the Functional and Structural Integrity of Photosystem II

Willem Vermaas, Jeroen Charité, Gaozhong Shen

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Two D2 mutants were created with a site-directed mutation near the presumable binding site of QA. In one of the mutants, in which Trp-253, the aromatic residue potentially involved in facilitating electron transport from pheophytin to QAand/or in binding of QA, had been replaced by Leu, PS II was undetectable in thylakoids. This mutant is an obligate photoheterotroph. In another mutant the Gly-215 residue, located next to the His residue that is proposed to bind QA and Fe2+, was mutated to Trp. This mutation leads to a rapid inactivation of oxygen evolution capacity in the light, and to a virtual elimination of the potential to grow photoautotrophically, but does not greatly affect the number of photosystem II reaction centers on a chlorophyll basis. We propose that proper binding of QA to the photosystem II reaction center complex is a prerequisite for stability of the photosystem II complex. Impairment of QA binding leads to rapid inactivation of photosystem II, which may be followed by a structural disintegration of the complex.

Original languageEnglish (US)
Pages (from-to)359-365
Number of pages7
JournalZeitschrift fur Naturforschung. C, Journal of biosciences
Volume45
Issue number5
DOIs
StatePublished - 1990

Fingerprint

Photosystem II Protein Complex
Structural integrity
Pheophytins
Mutation
Thylakoids
Disintegration
Chlorophyll
Electron Transport
Binding Sites
Oxygen
Light

Keywords

  • Cyanobacteria
  • Photoinhibition
  • Photosynthesis
  • Plastoquinone
  • Site-Directed Mutagenesis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

QA Binding to D2 Contributes to the Functional and Structural Integrity of Photosystem II. / Vermaas, Willem; Charité, Jeroen; Shen, Gaozhong.

In: Zeitschrift fur Naturforschung. C, Journal of biosciences, Vol. 45, No. 5, 1990, p. 359-365.

Research output: Contribution to journalArticle

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