Purification and characterization of the B808-866 light-harvesting complex from green filamentous bacterium Chloroflexus aurantiacus

Yueyong Xin, Su Lin, Gabriel A. Montaño, Robert E. Blankenship

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

The integral membrane light-harvesting complex B808-866 from the thermophilic green filamentous bacterium Chloroflexus aurantiacus has been isolated and characterized. Reversed-phase HPLC analysis demonstrated that the number of bacteriochlorophyll (BChl) in the B808-866 antenna complex is 36 ± 2 per reaction center. The main carotenoid type is γ-carotene, and the molar ratio of BChl to carotenoid is 3:2. The steady-state absorption and fluorescence spectroscopy of the B808-866 complex are reminiscent of the well-studied LH2 peripheral antenna of purple bacteria, whereas the protein sequence and the circular dichroism spectrum of B808-866 is more similar to the LH1 inner core antenna. The efficiency of excitation transfer from carotenoid to BChl is about 25%. The above results combined with electron microscopy and dynamic light scattering analysis suggest that the B808-866 antenna is more like the LH1, whereas surrounds the reaction center but probably consists of 24 building blocks with a ring diameter of about 20 nm. The above results suggested that there are probably two reaction centers inside the ring of B808-866. The unique properties of this light-harvesting complex may provide insights on the protein-pigment interactions in bacterial photosynthesis.

Original languageEnglish (US)
Pages (from-to)155-163
Number of pages9
JournalPhotosynthesis research
Volume86
Issue number1-2
DOIs
StatePublished - Nov 1 2005

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Keywords

  • B808-866 light-harvesting complex
  • Characterization
  • Chloroflexus aurantiacus
  • Purification
  • Structure

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

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