Proximity-enhanced synthesis of DNA-peptide-DNA triblock molecules

Tara MacCulloch; Alexandra Novacek; Nicholas Stephanopoulos

doi:10.1039/d1cc04970d

Proximity-enhanced synthesis of DNA-peptide-DNA triblock molecules

Tara MacCulloch, Alexandra Novacek, Nicholas Stephanopoulos

Molecular Sciences, School of (SMS)

Research output: Contribution to journal › Article › peer-review

Abstract

We report a proximity-enhanced method to synthesize a peptide flanked by two different oligonucleotide handles. Our method relies on sequential bioorthogonal reactions, and partial hybridization of the second handle to the first. We demonstrate the synthesis of a protease-responsive DNA “latch” and a cyclic bioactive peptide using this method.

Original language	English (US)
Pages (from-to)	4044-4047
Number of pages	4
Journal	Chemical Communications
Volume	58
Issue number	25
DOIs	https://doi.org/10.1039/d1cc04970d
State	Published - Mar 2 2022

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/d1cc04970d

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title = "Proximity-enhanced synthesis of DNA-peptide-DNA triblock molecules",

abstract = "We report a proximity-enhanced method to synthesize a peptide flanked by two different oligonucleotide handles. Our method relies on sequential bioorthogonal reactions, and partial hybridization of the second handle to the first. We demonstrate the synthesis of a protease-responsive DNA “latch” and a cyclic bioactive peptide using this method.",

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AB - We report a proximity-enhanced method to synthesize a peptide flanked by two different oligonucleotide handles. Our method relies on sequential bioorthogonal reactions, and partial hybridization of the second handle to the first. We demonstrate the synthesis of a protease-responsive DNA “latch” and a cyclic bioactive peptide using this method.

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Proximity-enhanced synthesis of DNA-peptide-DNA triblock molecules

Abstract

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